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GF35L
Sigma-AldrichAnti-BDNF Mouse mAb (35928.11)
Anti-BDNF, mouse monoclonal, clone 35928.11, recognizes the ~20 kDa BDNF protein in brain extracts. It is validated for ELISA, Western blotting, immunohistochemistry, and neutralization studies.
More>>Anti-BDNF, mouse monoclonal, clone 35928.11, recognizes the ~20 kDa BDNF protein in brain extracts. It is validated for ELISA, Western blotting, immunohistochemistry, and neutralization studies. Less<<
Anti-BDNF Mouse mAb (35928.11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.
Catalogue Number
GF35L
Brand Family
Calbiochem®
Synonyms
Anti-Brain Derived Neurotrophic Factor
References
References
Riccio, A., et al. 1997. Science277 1097. Sendtner, M., et al. 1996. Neurochem. Res.21, 831. Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442. Gotz, R., et al. 1994. Nature372, 266. Snider, W.D. 1994. Cell77, 627. Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.
The full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogen
recombinant protein consisting of amino acids 129-247 of human BDNF
Immunogen
Human
Clone
35928.11
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
Anti-BDNF Mouse mAb (35928.11) Certificates of Analysis
Title
Lot Number
GF35L
References
Reference overview
Riccio, A., et al. 1997. Science277 1097. Sendtner, M., et al. 1996. Neurochem. Res.21, 831. Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442. Gotz, R., et al. 1994. Nature372, 266. Snider, W.D. 1994. Cell77, 627. Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Neurotrophic factors define a family of related proteins, which are important in the development, maintenance, and regulation of neurons in both the peripheral and central nervous system. In addition to the prototypical nerve growth factor (NGF), several other factors have been identified which interact with a family of tyrosine kinase receptors. TrkA is the kinase signaling receptor for NGF, while TrkB mediates signaling for both brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5). TrkC is the primary receptor for neurotrophin-3 (NT-3), although this neurotrophin can also activate TrkA and TrkB receptors under certain conditions. Axotomy-induced cell death is one experimental paradigm that has been used to discriminate between the effects of various neurotrophic factors on the survival of different neuronal populations. BDNF, NT-3, and NT-4/5 act as motor neuron survival factors, whereas NGF has no effect on the survival of spinal cord motor neurons. NGF is neuroprotective for cholinergic neurons in the central nervous system. Retrogradely transported NGF-TrkA has also been shown to signal the CREB transcription factor in sympathetic neurons. A putative new member of this gene family, neurotrophin-6 (NT-6), has also been identified from the fish Xephophorus. Like NGF, NT-6 promotes the survival of sympathetic and sensory neurons, albeit with a lower potency.
Host
Mouse
Immunogen species
Human
Immunogen
recombinant protein consisting of amino acids 129-247 of human BDNF
Clone
35928.11
Isotype
IgG₁
Species
human
Form
Lyophilized
Formulation
Lyophilized from PBS, 5% trehalose.
Preservative
None
Comments
The full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Riccio, A., et al. 1997. Science277 1097. Sendtner, M., et al. 1996. Neurochem. Res.21, 831. Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442. Gotz, R., et al. 1994. Nature372, 266. Snider, W.D. 1994. Cell77, 627. Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.