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MABS1193 Anti-ABCB11 Antibody, clone 10D4.1

Anti-ABCB11 Antibody, clone 10D4.1 is an antibody against ABCB11 for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-ABCB11 Antibody, clone 10D4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Liver Physiology Gene Symbol: ABCB11, BRIC2, BSEP, PFIC2, SPGP UniProt Number: O95342
MABS1193
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1193
DescriptionAnti-ABCB11 Antibody, clone 10D4.1
Alternate Names
  • Bile salt export pump
  • ATP-binding cassette sub-family B member 11
  • Sister of P-glycoprotein
Background InformationBile salt export pump (UniProt O95342; also known as ATP-binding cassette sub-family B member 11, Sister of P-glycoprotein) is encoded by the ABCB11 (also known as BRIC2, BSEP, PFIC2, SPGP) gene (Gene ID 8647) in human. Bile is produced by hepatocytes in the liver and released into the biliary system of ducts and gallbladder. After food ingestion, bile is released in the form of mixed micelles of salts and phospholipids into the duodenum where its detergent properties act to solubilize lipids to help their absorption by the intestine. Bile salt export pump is an ATP-binding cassette (ABC) family member localized to the hepatocyte canalicular membrane where it plays an important role in the bile salt-dependent bile flow and the normal enterohepatic circulation of bile salts from the distal intestine back to the liver. ABCB11/BSEP gene mutations are associated with progressive familial intrahepatic cholestasis type 2 (PFIC2), benign recurrent intrahepatic cholestasis type 2 (BRIC2), drug-induced cholestasis, hormone-dependent intrahepatic cholestasis of pregnancy (ICP), biliary lithiasis, and transient neonatal cholestasis, as a result of impaired bile flow. Bile salt export pump is a 12-transmembrane protein (a.a. 63-83, 148-168, 216-236, 241-261, 320-340, 354-374, 756-776, 795-815, 870-890, 891-911, 980-1000, 1012-1032) with both its N- and C-terminal ends exposed cyroplasmically (a.a. 1-62 and 1033-1321).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ABCB11 Antibody, clone 10D4.1 is an antibody against ABCB11 for use in Western Blotting, Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected ABCB11 in human liver tissue sections.
Biological Information
ImmunogenGST-tagged recombinant fragment derived from the fourth cytoplasmic domain of human ABCB11.
EpitopeThe fourth cytoplasmic domain.
Clone10D4.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 10D4.1 targets the fourth cytoplasmic domain of human ABCB11.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ABCB11
  • BRIC2
  • BSEP
  • PFIC2
  • SPGP
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~150-175 kDa observed. 146.4 kDa calculated. The multiple banding pattern observed is consistent with the detection of the target protein with varying degrees of glycosylation.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ABCB11 in 10 µg of HepG2 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1193 04055977333350