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NA02A Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate

This Anti-2,2,7-Trimethylguanosine Mouse mAb (K121) Agarose Conjugate is validated for use in Affinity Purification, IP, IP, ICC, Paraffin Sections for the detection of 2,2,7-Trimethylguanosine.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NA02A
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
NA02A-0.5ML Plastic ampoule 0.5 ml
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Description
OverviewRecognizes the exposed 5′ cap structure (2,2,7-trimethylguanosine) of small nuclear RNAs (snRNAs). Also recognizes 7-methylguanosine-capped mRNA.
Catalogue NumberNA02A
Brand Family Calbiochem®
References
ReferencesCasciola-Rosen, L.A., et al. 1994. J. Biol. Chem. 269, 30757-30760.
Krainer, A.R. 1988. Nuc. Acids Res. 16, 9415.
Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71.
Reddy, R. and Busch, H., 1988. Birnstiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 1.
Krainer, A.R. and Maniatis, T. 1985. Cell 42, 725.
Kramer, A., et al. 1984. Cell 38, 299.
Lerner, M.R., et al. 1980. Nature 283, 220.
Product Information
FormLiquid suspension
FormulationIn PBS.
Negative controlTransfer RNA (tRNA)
Positive controlHeterogenous nuclear RNA (hnRNA)
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesAffinity Purification Luhrmann, R. 1988. Birntiel, M.L. (ed) Springer-Verlag, Berlin and Heidelberg. 71. Immunoprecipitation Moteki, S. and Price, D. 2002. Mol. Cell 10, 599.
Key Applications Affinity Purification
Immunoblotting (Western Blotting)
Application NotesAffinity Purification (see application references)
Immunoprecipitation (15 µl beads/sample)
Immunoprecipitation (1-2 µg/ml; see application references)
Immunocytochemistry (use Cat. No. NA02)
Paraffin Sections (use Cat. No. NA02)
Application CommentsAlso recognizes 7-methylguanosine-capped mRNA. 2,2,7-trimethylguanosine (Ab-1) is human, mouse, and rat reactive. This antibody reacts with the exposed 5' cap structure of the snRNAs and the antibody can be used for the purification of snRNPs by immunoaffinity chromatography. All of the major snRNPs bind to the monoclonal antibody column and can be gently eluted by washing with 7-methyl guanosine. Antibody should be titrated for optimal results in individual sample types.
Biological Information
Immunogen2,2,7-trimethylguanosine
CloneK121
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Global Trade Item Number
Catalogue Number GTIN
NA02A-0.5ML 04055977227611