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SCR004 Alkaline Phosphatase Detection Kit

SCR004
100 assays  
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key Applications
Cult
Description
Catalogue NumberSCR004
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAlkaline Phosphatase Detection Kit
OverviewMillipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.
Materials Required but Not Delivered1. Fixative (e.g. 4% Paraformaldehyde in PBS)

2. 1 x Rinse Buffer (e.g. TBST: 20mM Tris-HCl, pH 7.4, 0.15M NaCl, 0.05% Tween-20)

3. Hematoxylin (optional)

4. Microscope
Product Information
Components
  • Fast Red Violet solution (0.8g/L stock) (Part No. 90239). Two 15mL bottles.
  • Napthol AS-BI phosphate solution (4mg/mL) in AMPD buffer (2mol/L), pH 9.5 (Part No. 90234). One 15mL bottle.
Quality LevelMQ100
Applications
ApplicationThis Alkaline Phosphatase Detection Kit is a specific & sensitive tool for the phenotypic assessment of Embryonic Stem (ES) cell differentiation by the determination of AP activity.
Key Applications
  • Stem Cell Culture
Application NotesEmbryonic stem (ES) cells are totipotent cells derived from the inner cell mass (ICM) of preimplantation mammalian embryos and are capable of unlimited, undifferentiated proliferation in vitro [1,2,3]. Undifferentiated murine ES cells can be maintained in vitro for extended periods in media containing the cytokine, leukemia-inhibitory factor (LIF) or Millipore's proprietary ES cell culture reagent, ESGRO [4,5]. The undifferentiated state of ES cells can be characterized by high level of expression of Alkaline Phosphatase (AP) [6], the expression of surface markers including SSEA and TRA antigens and the transcription factor Oct-4.

Millipore's Alkaline Phosphatase Detection Kit (Catalog number SCR004) is a specific and sensitive tool for the phenotypic assessment of ES cell differentiation by the determination of AP activity.

Available separately from Millipore are the monoclonal antibodies TRA-2-49 (Catalog Number MAB4349) and TRA-2-54 (Catalog Number MAB4354), which permit the detection of Liver / Bone / Kidney isozyme of AP as expressed by ES cells. When used in flow cytometry, these reagents permit a quantitative assessment of AP expression by ES cells [7].

For Research Use Only; Not for use in diagnostic procedures
Biological Information
Species Reactivity
  • Mammals
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Human Embryonic Stem Cells
  • Mesenchymal Stem Cells
  • Neural Stem Cells
  • Hematopoietic Stem Cells
  • Epithelial Cells
  • Pancreatic Stem Cells
  • Cardiac Stem Cells
  • Induced Pluripotent Stem Cells
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsThe Alkaline Phosphatase Detection Kit consists of two components used for determining AP activity.

When stored at 2-8º C, the kit components are stable up to the expiration date. Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date.
Packaging Information
Material Size100 assays
Global Trade Item Number
Catalogue Number GTIN
SCR004 04053252503757