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14-606M ASK1 Protein, active, 250 µg

Active, N-Terminal GST & C-terminal His6-tagged, recombinant, human ASK1 amino acids 649-946, for use in Kinase Assays.

ASK1 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-606M
250 µg  Also available in 10 μg size (cat#14-606) and in bulk (cat# 14-606-K).
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue Number14-606M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionASK1 Protein, active, 250 µg
OverviewN-Terminal GST and C-terminal His6-tagged, recombinant, human ASK1 amino acids 649-946
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-Terminal GST & C-terminal His6-tagged, recombinant, human ASK1 amino acids 649-946, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in E. coli cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryMitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Gene Symbol
  • MAP3K5
  • MEKK5
  • ASK1
  • ASK-1
  • MAPKKK5
Protein TargetAsk1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q99683 # Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
COFACTOR: Magnesium.
SIZE: 1374 amino acids; 154537 Da
SUBUNIT: Homodimer when inactive. Binds both upstream activators and downstream substrates in multimolecular complexes. Associates with and inhibited by HIV-1 Nef. Interacts with DAB2IP.
TISSUE SPECIFICITY: Abundantly expressed in heart and pancreas.
SIMILARITY: SwissProt: Q99683 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight61.5kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of MBP
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-606) and in bulk (cat# 14-606-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
14-606M 04053252277733

Related Products & Applications

Alternative Packsize

Catalogue Number Description
14-606 ASK1 Protein, active, 10 µg

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Categories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases