1-D separation of single protein digests on ProteoChrom® silica gel plates
A. ProteoChrom® HPTLC silica gel 60 F254s plate followed by fluorescamine staining

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 		 B. ProteoChrom® HPTLC silica gel 60 F254s plate followed by ninhydrin staining

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Sample volume A: 1.5 μl
B: 4 μl
Concentration 2 mg/mL
Application system Automatic TLC sampler 4 (CAMAG)
Mobile phase 2-butanol/pyridine/ammonia (25%) /water (39/34/10/26)
Migration distance 5 cm
Migration time 45 min
Staining/detection  A: Fluorescamine
B: Ninhydrin