Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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PF133
Sigma-AldrichTACE, His•Tag®, Human, Recombinant, T. ni.
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Description
Overview
Recombinant, human TACE (amino acids 1-671) fused to a His•Tag® sequence and expressed in T. ni. insect cells. The major form of the purified protein corresponds to the mature form with an apparent molecular mass of ~70 kDa.
Catalogue Number
PF133
Brand Family
Calbiochem®
Synonyms
ADAM17, Human α-Secretase
Product Information
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered 25 mM Tris-HCl, pH 8.0.
≥100 pmol/min/µg as measured by the cleavage of 10 µM of fluorescent substrate (MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH₂; Cat. No. 616402) in 100 µl of reaction buffer at 37°C.
Purity
≥90% by SDS-PAGE
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Avoid repeated freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
TACE, His•Tag®, Human, Recombinant, T. ni. Certificates of Analysis
Title
Lot Number
PF133
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
17-June-2008 RFH
Synonyms
ADAM17, Human α-Secretase
Description
Recombinant, human TACE (amino acids 1-671) His•Tag® sequence and expressed in T. ni. insect cells. The major form of the purified protein corresponds to the mature form with an apparent molecular mass of ~70 kDa.
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered 25 mM Tris-HCl, pH 8.0.
Purity
≥90% by SDS-PAGE
Biological activity
≥100 pmol/min/µg as measured by the cleavage of 10 µM of fluorescent substrate (MCA-Pro-Leu-Ala-Gln-Ala-Val-Dpa-Arg-Ser-Ser-Ser-Arg-NH₂; Cat. No. 616402) in 100 µl of reaction buffer at 37°C.
Solubility
Reconstitute with 100 µl dH₂O.
Preservative
None
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Avoid repeated freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
