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574601 Superoxide Dismutase Assay Kit II

Superoxide Dismutase Assay Kit II MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
574601
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574601-1KIT
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      Description
      OverviewA sensitive spectrophotometric assay (450 nm) for the measurement of all three types (Cu/Zn, Mn, and Fe) of SOD.
      Catalogue Number574601
      Brand Family Calbiochem®
      Materials Required but Not Delivered• Plate reader capable of reading absorbance at 450 nm.
      • Adjustable pipettors and a repeat pipettor.
      • A source of pure water. Glass distilled water or HPLC-grade water is acceptable.
      References
      ReferencesMaier, C.M. and Chan, P.H 2002. The Neuroscientist 8, 323.
      Mattiazz, M., et al. 2002. J. Biol. Chem. 277, 29626.
      Beckman, J.S. and Koppenol, W.H. 1996. Am. J. Physiol. 271, C1424.
      Liu, D. 1996. J. Mol. Neuro. 7, 159.
      MacMillan-Crow, L.A., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11853.
      Sun, E., et al. 1995. Biol. Trace Elem. Res. 48, 231.
      Sandstrom, J., et al. 1994. J. Biol. Chem. 269, 19163.
      Marklund, S. 1980. Acta Physiol. Scand. Suppl. 492, 19.
      Malstrom, B. 1975. in The Enzymes. Boyer, P., editor. XIIB, Academic Press, New York, 533.
      Product Information
      Unit of DefinitionThe amount of enzyme needed to exhibit 50% dismutation of the superoxide radical.
      Detection methodColorimetric
      Form96 Tests
      Format96-well plate
      Kit containsAssay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol.
      Positive controlBovine erythrocyte SOD (Cu/Zn)
      Quality LevelMQ100
      Biological Information
      Assay range0.025-0.25 units per ml SOD
      Assay time1.5 h
      Sample TypePlasma, serum, erythrocyte lysates, and other lysates, tissue homogenates, and cell lysates
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Storage ConditionsUpon arrival store entire contents of the kit at -20°C.
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsAssay Buffer, Sample Buffer, Radical Detector, SOD Standard, Xanthine Oxidase, 96-Well Plate, Plate Cover, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      574601-1KIT 07790788051822