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14-735 PDGFR α (550-end, V561D) Protein, active human, 10 µg

This V561D mutation has been found in some patients with gastrointestinal stromal tumors & confers constitutive activity.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-735
10 µg  
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      Overview

      Description
      Catalogue Number14-735
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPDGFR α (550-end, V561D) Protein, active human, 10 µg
      OverviewN-terminal 6His-tagged, recombinant, human PDGFR alpha, amino acids 550 - end, containing the V561D mutation. This V561D mutation has been found in some patients with gastrointestinal stromal tumors and confers constitutive activity (Hirota S et al., Gastroenterology (2003); 125:660-667 and Lasota, J. et al., Laboratory Investigation (2004): 84: 874-883).
      Alternate Names
      • Platelet-derived growth factor receptor alpha
      • PDGF-R-alpha
      • PDGFR-alpha
      • Alpha platelet-derived growth factor receptor
      • Alpha-type platelet-derived growth factor receptor
      • CD140 antigen-like family member A
      • CD140a antigen
      • Platelet-derived growth factor alpha receptor
      • Platelet-derived growth factor receptor 2
      • PDGFR-2
      • CD140a
      Product Information
      Presentation10 μg of recombinant protein in 100 μL of 50 mM Tris-HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationThis V561D mutation has been found in some patients with gastrointestinal stromal tumors & confers constitutive activity.
      Key Applications
      • Kinase Assay
      Biological Information
      PurityPurity 81% by SDS-PAGE and Coomassie blue staining.
      SourceExpressed by baculovirus in Sf21 cells.
      Specific Activity979 U/mg, where one unit of PDGFR alpha (V561D) activity is defined as 1nmol phosphate incorporated into 250 μM GGMEDIYFEFMGGKKK per minute at 30°C with a final ATP concentration of 100 μM.
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
      Gene Symbol
      • PDGFRA
      • CD140a
      • PDGF-R-alpha
      • MGC74795
      • PDGFR2
      • Rhe-PDGFRA
      • CD140A
      Protein TargetPDGFRα
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyTK
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P16234 # Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity.
      SIZE: 1089 amino acids; 122670 Da
      SUBUNIT: Homodimer, and heterodimer with PDGFRB. Interacts with SHB (By similarity).
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DISEASE: SwissProt: P16234 # A fusion of PDGFRA and FIP1L1 (FIP1L1-PDGFRA), due to an interstitial chromosomal deletion, is the cause of some cases of hypereosinophilic syndrome (HES) [MIM:607685]. HES is a rare hematologic disorder characterized by sustained overproduction of eosinophils in the bone marrow, eosinophilia, tissue infiltration and organ damage.
      SIMILARITY: SwissProt: P16234 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
      Molecular Weight63.5 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation 250 μM GGMEDIYFEFMGGKKK. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active PDGFR alpha.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStorage and Stability: Stable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
      Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
      Packaging Information
      Material Size10 µg
      Global Trade Item Number
      Catalogue Number GTIN
      14-735 04053252332326