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14-1121 MultiDsk-Ubiquitin-binding protein reagent

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14-1121
15 µL  
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      Overview

      Key Spec Table

      Key ApplicationsFormat
      APAPurified
      Description
      Catalogue Number14-1121
      DescriptionMultiDsk-Ubiquitin-binding protein reagent
      Alternate Names
      • Dsk-Ubiquitin-binding protein reagent
      • Ubiquitin domain-containing protein DSK2
      Background InformationMultiDsk - Ubiquitin-binding protein reagent is a GST fusion protein containing an array of five ubiquitin-associated (UBA) domains from the yeast Ub-binding protein Dsk2 (aa 327-373; UniProt P48510). MultiDsk binds and protects ubiquitinated proteins from deubiquitination. When immobilized on glutathione resins, MultiDsk can be used for affinity pull-down of mono- and poly-ubiquitinated proteins from cell extracts and tissue homogenates. This construct also contains a C-terminal 6xHis sequence and an internal 6xHis sequence between the N-terminal GST and the 5XUBA sequence (PMID 23056298). MultiDsk is expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956).
      Product Information
      FormatPurified
      PresentationPurified ubiquitin-binding protein in buffer containing 25mM Tris-HCl pH7.5, 150mM NaCl, 2mM β-mercaptoethanol with 50% glycerol without preservatives.
      Quality LevelMQ100
      Applications
      ApplicationThis MultiDsk-Ubiquitin-binding protein reagent is validated for use in Affinity Precipitation Assays for the pull-down of mono- and poly-ubiquitinated proteins.
      Key Applications
      • Affinity Precipitation Assay
      Application NotesAffinity Precipitation Assay Analysis: A representative lot of MultiDsk-Ubiquitin-binding protein was immobilized on agarose beads and used to precipitate ubiquitinated proteins from yeast and HEK293 whole cell extracts. (Wilson, D.M., et al. (2012) PLoS One. 7(10):e46398).
      Affinity Precipitation Assay Analysis: A representative lot of MultiDsk-Ubiquitin-binding protein was immobilized on agarose beads and used to pull down ubiquitinated proteins from yeast whole cell extracts. (Wilson, D.M., et al. (2013) Cell. 154:983-995).
      Biological Information
      ImmunogenThis is an protein reagent, not an antibody as it is a yeast protein, with a GST tag and a His tag, expressed in e. coli.
      EpitopeUbiquitin
      CloneN/A
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityMono- and poly-ubiquitinated proteins
      SourceYeast protein, with a GST tag and a His tag, expressed in e. coli.
      Gene Symbol
      • DSK2
      • SHE4
      • YMR276W
      • YM8021.02
      Purification MethodAffinity
      UniProt Number
      Molecular WeightMultiple bands observed.
      Product Usage Statements
      Quality AssuranceEvaluated by Affinity Precipitation in yeast whole cell extract.

      Affinity Precipitation Assay Analysis: Ubiquitinated proteins were isolated from 1.0 mg yeast whole cell extract by MultiDsk-Ubiquitin-binding protein reagent (7.8 ug to 31.3 ug MultiDsk per pull-down) immobilized on agarose beads
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -80°C from date of receipt.
      Shipped on Dry Ice. Upon delivery aliquot and store at -80°C. Avoid freeze / thaw cycle
      Packaging Information
      Material Size15 µL
      Global Trade Item Number
      Catalogue Number GTIN
      14-1121 04055977158922