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			96-Well Plate

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	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

CCYTMAG90KPX13BK MilliporeMILLIPLEX Canine Cytokine/Chemokine Magnetic Bead Panel - Premixed 13 Plex Space Saver (Bulk) Packaging

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in canine serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Technical Information
Protocols

Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology

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CCYTMAG90KPX13BK

96-well plate

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Overview

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Key Spec Table

Analytes Available	Species Reactivity	Key Applications	Detection Methods
GM-CSF IFN-γ IL-2 IL-6 IL-7 IL-8 IL-10 IL-15 IL-18 IP-10 KC-like MCP-1 TNF-α	Ca	Mplex	Luminex xMAP

Description
Catalogue Number	CCYTMAG90KPX13BK
Trade Name	MILLIPLEX
Description	MILLIPLEX Canine Cytokine/Chemokine Magnetic Bead Panel - Premixed 13 Plex Space Saver (Bulk) Packaging
Overview	In addition to studying inflammation for canine nutrition and disease research, the dog is an important animal model for human biomedical research and drug development. Millipore recognizes that the availability of canine-specific immunoassay products is limited. Therefore, the MILLIPLEX Canine Cytokine / Chemokine Panel enables you to focus on the potential role of cytokines in disease, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improving productivity.
Background Information	The immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis and cancer.

References

Product Information
Detection method	Luminex xMAP
Configuration	Premixed
Precision, %	Intra-assay: 2 - 4 Inter-assay: 8 - 17
Panel Type	MAGNETIC Cytokines/Chemokines
Quality Level	MQ200

Applications
Application	Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in canine serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	• This is an overnight incubation assay. • This assay requires 25 µL of a 1:3 dilution of plasma or serum, or 25 μL cell culture supernatant per well.

Biological Information
Specificity	UPDATE >> Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Canine
Analytes Available	GM-CSF IFN-γ IL-2 IL-6 IL-7 IL-8 IL-10 IL-15 IL-18 IP-10 KC-like MCP-1 TNF-α
Cross Reactivity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy	GM-CSF: 95% IFN-g: 94% KC-like: 91% IP-10: 94% IL-2: 96% IL-6: 98% IL-7: 102% IL-8: 101% IL-10: 95% IL-15: 109% IL-18: 96% MCP-1: 91% TNFa: 98%

Physicochemical Information
Sensitivity	GM-CSF: 9.2 pg/mL IFN-γ: 13.6 pg/mL IP-10: 3.2 pg/mL IL-2: 3.5 pg/mL IL-6: 3.7 pg/mL IL-7: 7.5 pg/mL IL-8: 21.7 pg/mL IL-10: 8.5 pg/mL IL-15: 9.0 pg/mL IL-18: 5.8 pg/mL KC-like: 5.3 pg/mL MCP-1: 21.0 pg/mL TNFα: 6.1 pg/mL
Standard Curve Range	All analytes 12.2 - 50,000 pg/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
CCYTMAG90KPX13BK	04055977277609

Documentation

Protocols

Title
Protocol- CCYTOMAG-90K\|CCYTMG-90K-PX13\|CCYTMAG90KPX13BK

Technical Info

Title
96 well Template 2 Up
96 well Template Single

Alternative Format

Catalogue Number

Description

CCYTMG-90K-PX13

MILLIPLEX Canine Cytokine/Chemokine Magnetic Bead Panel - Premixed 13 Plex - Immunology Multiplex Assay

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CCYTOMAG-90K

MILLIPLEX Canine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

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