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SCC412 LbetaT2 Mouse Pituitary Gonadotrope Cell Line

The LbetaT2 cell line has been widely used as an in vitro model for the study of gonadotropin gene regulation and GnRH signaling

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SCC412
≥1X10⁶ cells/vial   
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCC412
      DescriptionLbetaT2 Mouse Pituitary Gonadotrope Cell Line
      OverviewGonadotropin-releasing hormone (GnRH) plays a key role in the control of reproduction in mammals. GnRH acts via its receptor, GnRH-R, to trigger the synthesis and release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) by the pituitary gonadotropes. In turn, the gonadotropins regulate gametogenesis and steroidogenesis in the gonads. LH and FSH comprise a common glycoprotein hormone  subunit, CGA, and the respective specific  subunits LH and FSH. The individual  subunits bind noncovalently to the common a subunit to form the mature glycoprotein hormones. Lhb gene expression is preferentially induced by high-frequency GnRH pulses, whereas low-frequency pulses favor Fshb expression.
      Alternate Names
      • L-beta-T2
      • L beta T2
      • L(beta)T2
      • Lbetat2
      Background InformationGonadotropin-releasing hormone (GnRH) plays a key role in the control of reproduction in mammals. GnRH acts via its receptor, GnRH-R, to trigger the synthesis and release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) by the pituitary gonadotropes. In turn, the gonadotropins regulate gametogenesis and steroidogenesis in the gonads. LH and FSH comprise a common glycoprotein hormone  subunit, CGA, and the respective specific  subunits LH and FSH. The individual  subunits bind noncovalently to the common a subunit to form the mature glycoprotein hormones. Lhb gene expression is preferentially induced by high-frequency GnRH pulses, whereas low-frequency pulses favor Fshb expression.
      Source
      The immortalized LT2 mouse pituitary gonadotrope cell line was generated by targeted tumorigenesis in transgenic mice carrying the rat LHb regulatory region linked to the SV40 T-antigen oncogene.1 LT2 cells express CGA, GnRH-R, and LHb. The cell line responds to pulsatile GnRH stimulation by upregulating Lhb and Gnrhr and secreting LH. The LT2 cell line has been widely used as an in vitro model for the study of gonadotropin gene regulation and GnRH signaling.2,3,4,5 The global mRNA expression profile and a genome-wide atlas of accessible chromatin in GnRH-stimulated LβT2 cells has recently been described6 and the STR profile has also been reported.7

      References
      Note: Please include the appropriate references from 1-5 in your publications that utilize them.
      1. Alarid ET, Windle JJ, Whyte DB, Mellon PL. Development 1996; 122(10):3319-3329.
      2. Turgeon JL, Kimura Y, Waring DW, Mellon PL. Molecular Endocrinology 1996; 10(4):439-450.
      3. Thomas P, Mellon PL, Turgeon J, Waring DW. Endocrinology 1996; 137(7):2929-2989.
      4. Alarid ET, Holley S, Hayakawa M, Mellon PL. Mol Cell Endocrinol 1998; 140(1-2):25-30.
      5. Pernasetti F, Vasilyev VV, Rosenberg SB, Bailey JS, Huang HJ, Miller WL, Mellon PL. Endocrinology 2001; 142(6):2284-2295.
      6. Ruf-Zamojski F et al. Front Endocrinol (Lausanne) 2018; 9:34.
      7. Ruf-Zamojski F et al. J Endocr Soc 2019; 3(5):902–920.
      References
      Product Information
      Applications
      ApplicationThe LbetaT2 cell line has been widely used as an in vitro model for the study of gonadotropin gene regulation and GnRH signaling
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
      Biological Information
      Cell Line Type
      • Cancer Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance Each vial contains ≥ 1X10⁶ viable cells.

      Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River
      Animal Diagnostic Services.

      Cells are verified to be of mouse origin and negative for inter-species contamination from rat,
      Chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a
      Contamination Clear panel by Charles River Animal Diagnostic Services

      Cells are negative for mycoplasma contamination.
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCC412 04065266024074