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71187 Insect PopCulture® Reagent

Protein extraction directly from insect cell cultures

Insect PopCulture® Reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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71187
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Overview

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71187-3
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      		 Plastic ampoule 50 ml
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      71187-4
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          		 Plastic ampoule 250 ml
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          Insect PopCulture® Reagent is a detergent-based lysis reagent specifically formulated for total insect cell culture extraction without the need for centrifugation. The improved method recovers both protein released into the medium and intracellular protein, increasing processing efficiency and target protein yields (Loomis 2002). It is amenable for automated expression-level screening and is fully compatible with the Ni-NTA His•Bind® affinity purification method. Insect PopCulture reagent can be used for protein extraction from insect cells grown in suspension and adherent cells grown on tissue culture plates.



          Description

          Catalogue Number71187 Brand Family Novagen®
          Features and benefits
          • No need to separate cells from culture media
          • No need to clarify cell extracts prior to purification
          • Higher protein yield due to target protein recovery from both medium and cells
          • Direct affinity adsorption of target proteins to IMAC affinity resin from the total culture extract
          • Compatible with protease inhibitor cocktails
          • Ideal for high-throughput, expression-level screening and protein purification
          • Compatible with transfected and baculovirus-infected insect cell cultures
          References
          ReferencesLoomis, K., et al. 2002. inNovations 15, 16.
          Product Information
          DeclarationThis product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage +15°C to +30°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          71187-3 04055977254075
          71187-4 04055977254082