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EZHIASF-14K Human Insulin ELISA, animal serum free

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EZHIASF-14K
96-well strip plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Insulin HELISAFluorescent Colorimetric
      Description
      Catalogue NumberEZHIASF-14K
      DescriptionHuman Insulin ELISA, animal serum free
      Background InformationThis unique Human Insulin Elisa kit is animal serum free–the monoclonal antibodies are harvested from clones grown in culture medium devoid of any animal protein.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: Coefficient of Variation:6.0–7.1
      • Intra-assay: Coefficient of Variation: 1.7–1.9
      Quality LevelMQ200
      Applications
      ApplicationThis Human Insulin ELISA, animal serum free is used to measure & quantify Insulin levels in Metabolism & Endocrine research.
      Key Applications
      • ELISA
      Application Notes3.5 hour assay, room temperature, 20 µL sample volume
      Biological Information
      SpecificityNo Cross-reactivity with Human Pro-insulin or Human C-peptide
      Species Reactivity
      • Human
      Analytes Available
      • Insulin
      Entrez Gene Number
      Entrez Gene SummaryAfter removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.
      Gene Symbol
      • INS
      • proinsulin
      • insulin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01308 # Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
      SIZE: 110 amino acids; 11981 Da
      SUBUNIT: Heterodimer of a B chain and an A chain linked by two disulfide bonds.
      SUBCELLULAR LOCATION: Secreted.
      DISEASE:SwissProt: P01308 # Defects in INS are the cause of familial hyperproinsulinemia [MIM:176730].
      SIMILARITY: SwissProt: P01308 ## Belongs to the insulin family.
      Accuracy
      • 88–128%
      Physicochemical Information
      Sensitivity
      • 0.73 µU/mL
      Linearity of Dilution73–100%
      Standard Curve Range
      • 2–200 µU/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      EZHIASF-14K 04053252588013

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      Categories

      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits