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EZHIL8-100K Human IL-8 Conferma™ ELISA

This Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

Human IL-8 Conferma™ ELISA MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: ELISA Detection Methods: Colorimetric Research Category: Inflammation & Immunology, Immune Response, Metabolism Research Sub-Category: Cytokines & Cytokine Receptors, Immune Response, Inflammation, Metabolism
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EZHIL8-100K
96-well strip plate  
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      Overview

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      IL-8 HELISAColorimetric
      Description
      Catalogue NumberEZHIL8-100K
      DescriptionHuman IL-8 Conferma™ ELISA
      Background InformationIL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
      Product Information
      Detection methodColorimetric
      Precision, %
      • Inter-assay: <4.7% CV
      • Intra-assay: <4.0% CV
      Quality LevelMQ200
      Applications
      ApplicationThis Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.
      Key Applications
      • ELISA
      Application Notes3 hour 45 minute assay, room temperature, 50µl of neat serum or plasma is recommened.
      Biological Information
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Analytes Available
      • IL-8
      Entrez Gene Number
      Entrez Gene SummaryIL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
      Gene Symbol
      • IL-8
      • NAF
      • GCP1
      • LECT
      • LUCT
      • NAP1
      • GCP-1
      • LYNAP
      • MDNCF
      • MONAP
      • NAP-1
      • SCYB8
      UniProt Number
      UniProt SummaryIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
      Accuracy
      • Serum 70%-105% Av 94%
      • Plasma 77%-102% Av 85%
      Physicochemical Information
      Linearity of DilutionSerum Linearity: 94-106% Serum Parallelism: 109% Plasma Linearity: 105%
      Standard Curve Range
      • 0.82pg/ml-200pg/ml
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Packaging Information
      Material Size96-well strip plate
      Global Trade Item Number
      Catalogue Number GTIN
      EZHIL8-100K 04065265645669