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EZHIL8-100K Human IL-8 Conferma™ ELISA

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EZHIL8-100K
96-well strip plate  
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      Overview

      Replacement Information

      Key Spec Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      IL-8 HELISAColorimetric
      Description
      Catalogue NumberEZHIL8-100K
      DescriptionHuman IL-8 Conferma™ ELISA
      Background InformationIL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
      References
      Product Information
      Detection methodColorimetric
      HS Code3002 15 90
      Precision, %
      • Inter-assay: <4.7% CV
      • Intra-assay: <4.0% CV
      Quality LevelMQ200
      Applications
      ApplicationThis Human Interleukin-8 (IL-8) ELISA kit is used for the quantification of Human IL-8 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.
      Key Applications
      • ELISA
      Application Notes3 hour 45 minute assay, room temperature, 50µl of neat serum or plasma is recommened.
      Biological Information
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Analytes Available
      • IL-8
      Entrez Gene Number
      Entrez Gene SummaryIL-8 is secreted by mononuclear macrophages, neutrophils, eosinophils, T lymphocytes, epithelial cells, and fibroblasts. It functions as a chemotactic factor by guiding the neutrophils to the site of infection. IL-8 also participates with other cytokines in the proinflammatory signaling cascade. The protein is implicated in numerous pathologies including, bacterial, cancer, cardiovascular and viral diseases.
      Gene Symbol
      • IL-8
      • NAF
      • GCP1
      • LECT
      • LUCT
      • NAP1
      • GCP-1
      • LYNAP
      • MDNCF
      • MONAP
      • NAP-1
      • SCYB8
      UniProt Number
      UniProt SummaryIL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
      Accuracy
      • Serum 70%-105% Av 94%
      • Plasma 77%-102% Av 85%
      Physicochemical Information
      Linearity of DilutionSerum Linearity: 94-106% Serum Parallelism: 109% Plasma Linearity: 105%
      Standard Curve Range
      • 0.82pg/ml-200pg/ml
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      EZHIL8-100K 04065265645669

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits