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251500 DAF-2 - CAS 205391-01-1 - Calbiochem

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

DAF-2 - CAS 205391-01-1 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 4,5-Diaminofluorescein

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251500
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
205391-01-1C₂₀H₁₄N₂O₅

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251500-1MG
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      Description
      OverviewA highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).
      Catalogue Number251500
      Brand Family Calbiochem®
      Synonyms4,5-Diaminofluorescein
      References
      ReferencesBerkels, R., et al. 2000. Cell Calcium 27, 281.
      Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
      Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
      Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
      Product Information
      CAS number205391-01-1
      ATP CompetitiveN
      DeclarationManufactured by Daiichi Pure Chemicals Co., Ltd
      FormColorless to pale yellow solution
      FormulationA 5 mM (1 mg in 550 µl) solution in DMSO.
      Hill FormulaC₂₀H₁₄N₂O₅
      Chemical formulaC₂₀H₁₄N₂O₅
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetFluorescent indicator for the direct detection of nitric oxide (NO) in vitro.
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/38

      Irritating to eyes and skin.
      S PhraseS: 26

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      251500-1MG 04055977199222

      Documentation

      DAF-2 - CAS 205391-01-1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      DAF-2 - CAS 205391-01-1 - Calbiochem Certificates of Analysis

      TitleLot Number
      251500

      References

      Reference overview
      Berkels, R., et al. 2000. Cell Calcium 27, 281.
      Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
      Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
      Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-May-2020 JSW
      Synonyms4,5-Diaminofluorescein
      DescriptionA highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.
      FormColorless to pale yellow solution
      FormulationA 5 mM (1 mg in 550 µl) solution in DMSO.
      CAS number205391-01-1
      Chemical formulaC₂₀H₁₄N₂O₅
      Structure formulaStructure formula
      Purity≥98% by HPLC
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesBerkels, R., et al. 2000. Cell Calcium 27, 281.
      Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
      Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
      Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.