Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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234403
Sigma-AldrichComplement C3-Depleted Serum, Human
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Description
Overview
Human serum that has been depleted of complement component C3.
Catalogue Number
234403
Brand Family
Calbiochem®
References
Product Information
Unit of Definition
<b>Classical Pathway Activity:</b> One C3H50 unit measured by classical pathway activation is defined as the amount of C3 required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C3-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.
Form
Clear, straw colored liquid
Formulation
In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2.
Applications
Biological Information
Source
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Concentration Label
Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
≤ -70°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
Complement C3-Depleted Serum, Human Certificates of Analysis
Title
Lot Number
234403
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
09-June-2008 RFH
Description
Human serum that has been depleted of complement component C3.
Form
Clear, straw colored liquid
Formulation
In 150 mM NaCl, 50 mM sodium phosphate, pH 7.2.
Concentration Label
Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Classical Pathway Activity
Recommended volume C3-Dpl serum per assay: ≤30 µl
C3H50 units/mg purified C3 at the recommended input of C3-Dpl: ≥2000 units/mg
Ratio: ≥0.60 (C3H50/mg C3)/(C3H50/mg C3 in NHS Std)
C3D Reconstituted With C3
Ratio: ≥0.6 (CH50/ml C3-Dpl + C3)/(CH50/ml NHS Std)
Source
Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Unit definition
Classical Pathway Activity: One C3H50 unit measured by classical pathway activation is defined as the amount of C3 required to yield 50% lysis of 3 x 10⁷ EA when incubated in the presence of the recommended volume of C3-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++.
Storage
Avoid freeze/thaw ≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-70°C).
