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17-630 ChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set

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17-630
25 assays  25 assays per set. ~4 μL per chromatin immunoprecipitation
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      Overview

      Key Spec Table

      Species ReactivityKey Applications
      H, EuWB, Mplex, Cell Function Assay, ChIP
      Description
      Catalogue Number17-630
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ Acetyl-Histone H4 - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ Acetyl-Histone H4 set includes purified rabbit polyclonal antiserum and the normal rabbit IgG antiserum, which can be used to demonstrate that the acetyl-histone H4 antibody is capable of precipitating acetyl-histone H4 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product.
      Product Information
      FormatSerum
      Control
      • Included negative control rabbit IgG antiserum and control primers specific for human GAPDH promoter.
      PresentationAnti-Acetyl-Histone H4 (rabbit polyclonal IgG). One vial containing 100 μL of antiserum containing 0.05% sodium azide. Store at -20°C.

      Normal Rabbit Serum. One vial containing 100 uL antiserum containing 0.05% sodium azide. Store at -20°C.

      Control Primers. One vial containing 75 μL of 5 μM of each primer specific for for human GAPDH.
      Quality LevelMQ100
      Applications
      ApplicationThis ChIPAb+ Acetyl-Histone H4 -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Western Blotting
      • Multiplexing
      • Cell Function Assay
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blot Analysis:
      Acidextracted proteins from normal HeLa cells (Lane 1) and HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetyl Histone H4 (1:2000).
      Proteins were visualized using a goat-anti rabbit secondary antibody conjugated to HRP and a chemi-luminescence detection system. (Please see figures).
      Biological Information
      ImmunogenThe acetyl-histone H4 antiserum is made against a peptide corresponding to amino acids 2-19 of Tetrahymena histone H4.
      HostRabbit
      SpecificityRecognizes acetylated histone H4 of approximately 10 kDa. Cross-reacts with acetylated histone H2B from Tetrahymena and weakly crossreacts with acetylated histone H2B from HeLa cells.
      May cross-react with other acetylated proteins.
      Species Reactivity
      • Human
      • Eukaryote
      Species Reactivity NoteProven to react with human samples. Predicted broad cross-reactivity among eukaryotes based upon sequence similarity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      UniProt Number
      UniProt SummaryFunction: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
      Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
      Subcellular location: Nucleus.
      Post-translational modification: Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
      Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
      Monomethylation at Arg-4 (H4R3me1) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6.
      Symmetric dimethylation on Arg-4 (H4R3me2a) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
      Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
      Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
      Sumoylated, which is associated with transcriptional repression.
      Sequence similarities: Belongs to the histone H4 family.

      Molecular Weight10 kDa
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated chromatin prepared from untreated HeLa cells (1 X 106 cell equivalents) was subjected to chromatin immunoprecipitation using 4 μL of either a normal rabbit antiserum or Anti-Acetyl Histone
      H4 serum and the Magna ChIP A (Cat. # 17-610) Kit. Successful immunoprecipitation of acetyl histone H4 associated DNA fragments was verified by qPCR using control ChIP Primers flanking the human GAPDH promoter (Please see figures).
      Please refer to the EZ-Magna A ChIP™ (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. ~4 μL per chromatin immunoprecipitation
      Global Trade Item Number
      Catalogue Number GTIN
      17-630 04053252672538