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07-890-I Anti-phospho PTEN (Ser385) Antibody

This Anti-phospho PTEN (Ser385) Antibody is validated for use in Western Blotting and Peptide Inhibition Assay for the detection of phospho PTEN (Ser385).

Anti-phospho PTEN (Ser385) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PTEN, MMAC1, TEP1 UniProt Number: P60484
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07-890-I
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-890-I
      DescriptionAnti-phospho PTEN (Ser385) Antibody
      Alternate Names
      • Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
      • phospho PTEN (Ser385)
      • Mutated in multiple advanced cancers 1
      • Phosphatase and tensin homolog
      Background InformationPhosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (EC 3.1.3.16, EC 3.1.3.48, EC 3.1.3.67; UniProt P60484; also known as Mitochondrial PTENalpha, Phosphatase and tensin-like protein, Mutated in multiple advanced cancers 1, Mitochondrial phosphatase and tensin protein alpha, MMAC1 phosphatase and tensin homolog deleted on chromosome 10, Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN) is encoded by the PTEN (also known as 10q23del, BZS, CWS1, DEC, GLM2, TEP1, MMAC1, MHAM, PTEN1) gene (Gene ID 5728) in human. PTEN protein catalyzes the removal of phosphate from phosphoserine, phosphothreonine, and phosphotyrosine residues on substrate proteins, as well as the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate. PTEN plays a major role in controlling cell growth and proliferation, and PTEN gene mutains are found in a large number of cancers at high frequency. PTEN activity, stability, localization, and conformation are controlled through complex regulatory mechanisms, including the reversible phosphorylations of several Ser/Thr (S/T) residues in its unfolded C-terminal tail region (PTEN-Ctail, residues 351–403). PTEN S370, S380, T382, T383 and S385 are known target sites by casein kinase II (CK2), while S361, T363, S362, and T366 are phosphorylated by glycogen synthase kinase-3 β (GSK3β). In addition, Polo-like kinase 1 (Plk1), Plk3, the microtubule associated serine/threonine (MAST) kinase family, the serine/threonine kinase 11 (STK11, LKB1), and the casein kinase 1 (CK1) have also been reported to phosphorylate PTEN.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho PTEN (Ser385) Antibody is validated for use in Western Blotting and Peptide Inhibition Assay for the detection of phospho PTEN (Ser385).
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: Target specificity of this antibody was confirmed by antibody blocking using the immunogen peptide and the corresponding non-phosphorylated peptide prior to Western blotting detection of Ser385 phosphorylated C124S PTEN mutant in lysate from transfected MDA-MB-468 cells. (Lysate courtesy of Dr James Hastie, University of Dundee, UK).
      Biological Information
      ImmunogenPeptide corresponding to amino acid region encompassing the human, mouse, and rat phospho-PTEN (Ser385).
      EpitopeNear C-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityPTEN phosphorylated on Serine residue 385
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PTEN
      • MMAC1
      • TEP1
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~54 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MDA-MB-468 cells transfected with PTEN mutant (C124S) cell lysate.

      Western Blotting Analysis: This antibody (0.2 µg/mL) detected Ser385 phosphorylation of the dual lipid and protein phosphatase dead C124S PTEN mutant in lysates of C124S PTEN-transfected MDA-MB-468 cells (Lysate courtesy of Dr James Hastie, University of Dundee, UK).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-890-I 04055977294309

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies