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ABT282 Anti-phospho-NUMA1 Antibody (Thr2055)

This Anti-phospho-NUMA1 Antibody (Thr2055) is validated for use in Western Blotting and Peptide Inhibition Assay for the detection of phospho-NUMA1 (Thr2055).

Anti-phospho-NUMA1 Antibody (Thr2055) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: NUMA1, NUMA UniProt Number: Q14980
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ABT282
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT282
      DescriptionAnti-phospho-NUMA1 Antibody (Thr2055)
      Alternate Names
      • Nuclear mitotic apparatus protein 1
      • NuMA protein
      • SP-H antigen
      Background InformationNUMA1 or NUMA protein is an interesting and major component of the nuclear matrix of eukaryotic cells. NUMA1 is a highly abundant protein component that appears to serve a non-structural role but is needed for mitotic spindle poles and centrosome formation as well as a linker of microtubules. These functions are important for proper cytokinesis and the setup of the proper polarity axis during cell division. NUMA1 resides in the nuclear matrix during interphase. NUMA1 dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via a dynein/dynamin complex, and it remains there until the anaphase onset. Thus NUMA1 then can be found in the nucleus during cell rest and cytoplasmically and centrosome localized during cytokinesis. NUMA1 phosphorylation occurs by CDK1 and phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-phospho-NUMA1 Antibody (Thr2055) is validated for use in Western Blotting and Peptide Inhibition Assay for the detection of phospho-NUMA1 (Thr2055).
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Analysis: A 1:500 dilution from a representative lot blocked NUMA1 phosphorylated at Thr2055 in Hydroxyurea treated HeLa nuclear extract.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the tail region of human NUMA1 phosphorylated at Thr2055.
      EpitopeTail Region
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse, Rat, Bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NUMA1
      • NUMA
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~230 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract and Hydroxyurea treated HeLa nuclear extract.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected NUMA1 phosphorylated at Thr2055 in 10 µg of HeLa nuclear extract and Hydroxyurea treated HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT282 04055977039481

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies