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MABC1824-25UL Anti-phospho-MYO10 (Ser1060/1062/1066) Antibody, clone 2C10-6

Anti-phospho-MYO10 (Ser1060/1062/1066), clone 2C10-6, Cat. No. MABC1824, is a mouse monoclonal antibody that detects MYO10 phosphorylated on Serine 1060/1062/1066 and is tested for use in Immunocytochemistry and Western Blotting.

MABC1824-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1824-25UL
      DescriptionAnti-phospho-MYO10 (Ser1060/1062/1066) Antibody, clone 2C10-6
      Alternate Names
      • Unconventional myosin-X
      • Unconventional myosin-10
      Background InformationUnconventional myosin-X (UniProt: Q9HD67; also known as Unconventional myosin-10, MYO10) is encoded by the MYO10 (also known as KIAA0799) gene (Gene ID:4651) in human. Myosins are highly conserved, ubiquitously expressed proteins that form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Along with actin they serve as motor molecules with ATPase activity that is essential for muscle contraction. MYO10, a multi-domain protein, is ubiquitously expressed and is present as a monomer in the cytosol in an inactive conformation. However, it is homodimeric in its active, membrane-bound conformation. It interacts strongly with calmodulin 3 (CALM3) and this interaction is essential for its function in filopodial extension and motility. It contains a MyTH4 and an FERM domain at its C-terminal, which are required for its binding to tubulin and β-integrin, respectively. The N-terminal region contains a sequence (aa 619-641) that is required for its bind to actin. Its myosin motor domain is localized in amino acids 63-739. MYO10 is reported to promote tumor growth through inducing genomic instability and chronic inflammation in cancer cells. It regulates genomic instability, at least partially, through mediating mitotic progression. MYO10 is reported to contain a Degron motif, a short stretch of amino acids that drives the degradation of unstable protein. In addition, phosphorylation of serine residues in MYO10 (serine 1060, 1062, 1066) drives its β-TrCP1-dependent degradation. Phosphorylated form of MYO10 is shown to be less stable. Degron mutation are reported to abolish MYO10 phosphorylation, degradation, and mitotic regulation. Cancer cells expressing high levels or stabilized MYO10 display higher sensitivity to Taxol. Deletion of Degron motif results in complete stability of MYO10. (Ref.: Pozo, MF., et al. (2023). Cell Rep. 42(5); 112531; Pozo, MF., et al. (2021). Sci Adv. 7(38); eabg6908).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-phospho-MYO10 (Ser1060/1062/1066), clone 2C10-6, Cat. No. MABC1824, is a mouse monoclonal antibody that detects MYO10 phosphorylated on Serine 1060/1062/1066 and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesTested applications

      Immunocytochemistry Analysis: A representative lot detected p-MYO10 (Ser1060/1062/1066) in Immunocytochemistry application (Pozo, F. M., et al. (2021). Sci Adv. 7(38); eabg6908).

      Western Blotting Analysis: A representative lot detected p-MYO10 (Ser1060/1062/1066) in Western Blotting application (Pozo, F. M., et al. (2021). Sci Adv. 7(38); eabg6908).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserines 1060, 1062, and 1066 from human MYO10.
      EpitopeInternal
      Clone2C10-6
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2C10-6 is a mouse monoclonal antibody that detects MY10 phosphorylated on serine 1060, 1062, and 1066.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MYO10
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~260 kDa observed with GFP-MYO10; 237.35 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate U20S cells transfected with GFP-MYO10.

      Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected phospho-MYO10 (Ser 1060/1062/1066) in U20S cells stably transfected with GFP-MYO10, but not in lysate from cells with MYO10 knockout.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1824-25UL 04065269802433