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MABN722 Anti-WIF1 Antibody, clone 1G5

Anti-WIF1 Antibody, clone 1G5 is a highly specific mouse monoclonal antibody, that targets Wnt inhibitory factor 1 & has been tested in western blotting, IHC & Immunofluorescence.

Anti-WIF1 Antibody, clone 1G5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: WIF-1, WIF UniProt Number: Q9Y5W5
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MABN722
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IHC, IFMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN722
      DescriptionAnti-WIF1 Antibody, clone 1G5
      Alternate Names
      • WIF-1
      • WIF
      • Wnt inhibitory factor 1
      • WIF1
      Background InformationWIF1, also known as Wnt inhibitory factor 1 or WIF-1, is a secreted protein that binds to Wnt proteins and inhibits their activity. WNT proteins have been shown to act as an extracellular signaling molecules and may be involved in the control of embryonic development. WIf1 contains an N-terminal WIF domain and 5 epidermal growth factor (EGF)-like domains. It has been implied in recent sutyes that WIF1 expression is downregulated in many types of cancers.
      References
      Product Information
      FormatAscites
      Control
      • HeLa, NIH/3T3 and NTERA-2 cell lysates
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-WIF1 Antibody, clone 1G5 is a highly specific mouse monoclonal antibody, that targets Wnt inhibitory factor 1 & has been tested in western blotting, IHC & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected WIF1 in HeLa cells.

      Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected WIF1 in human ovarian tumor and lung cancer tissues.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human WIF1 expressed in E. Coli.
      Clone1G5
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • WIF-1
      • WIF
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~43 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa, NIH/3T3 and NTERA-2 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected WIF1 in HeLa, NIH/3T3 and NTERA-2 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN722 04053252918568