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CP74 Anti-Vinculin Mouse mAb (V284 or VLN01)

This Anti-Vinculin Mouse mAb (V284 or VLN01) is validated for use in Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Vinculin.

Anti-Vinculin Mouse mAb (V284 or VLN01) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP74
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Pricing & Availability

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CP74-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins in skin tissue.
      Catalogue NumberCP74
      Brand Family Calbiochem®
      Application Data
      Detection of human vinculin by immunoblotting. Sample: Human vinculin. Primary antibody: Anti-Vinculin Mouse mAb (V284 or VLN01) (Cat. No. CP74) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesPalovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961.
      Bakolitsa, C., et al. 1999 Cell 99, 603.
      Rudiger, M., et al. 1998 Bioessays 20, 733
      Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155
      Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1.
      Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131
      Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Negative controlNormal mouse serum
      Positive controlSkin cells or tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml, chemiluminescence)
      Immunoprecipitation (2 µg/ml lysate)
      Paraffin Sections (4-8 µg/ml, heat pre-treatment required)
      Application CommentsThis antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogensemi-purified, human vinculin
      ImmunogenHuman
      CloneV284 or VLN01
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP74-100UG 04055977221022

      Documentation

      Anti-Vinculin Mouse mAb (V284 or VLN01) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Vinculin Mouse mAb (V284 or VLN01) Certificates of Analysis

      TitleLot Number
      CP74

      References

      Reference overview
      Palovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961.
      Bakolitsa, C., et al. 1999 Cell 99, 603.
      Rudiger, M., et al. 1998 Bioessays 20, 733
      Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155
      Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1.
      Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131
      Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      ApplicationImmunoblotting (1 µg/ml, chemiluminescence)
      Immunoprecipitation (2 µg/ml lysate)
      Paraffin Sections (4-8 µg/ml, heat pre-treatment required)
      Application Data
      Detection of human vinculin by immunoblotting. Sample: Human vinculin. Primary antibody: Anti-Vinculin Mouse mAb (V284 or VLN01) (Cat. No. CP74) (1 µg/ml). Detection: chemiluminescence.
      DescriptionProtein G purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myeloma SP2/0 cells. Recognizes the ~130 (vinculin) and ~150 kDa (meta-vinculin) proteins.
      BackgroundVinculin is an attachment protein involved in the indirect binding of intracellular actin filaments to extracellular fibronectin. Vinculin binds to actin capping protein and α-actinin, which in turn binds to actin. Also, vinculin binds to talin, which in turn binds to the intracellular domain of the fibronectin receptor. Vinculin has been associated with tumor suppressor activity. Vinculin is widely distributed in tissues and expressed where smooth muscle actin and fibroblasts attach to the extracellular matrix. Vinculin has been found in all adherens junctions, while the larger splice variant, meta-vinculin, is found only in smooth and cardiac muscle.
      HostMouse
      Immunogen speciesHuman
      Immunogensemi-purified, human vinculin
      CloneV284 or VLN01
      IsotypeIgG₁
      Specieshuman
      Positive controlSkin cells or tissue
      Negative controlNormal mouse serum
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody is useful for studies of the role of vinculin in platelet activation. Vinculin (Ab-1) exhibits membrane and cytoplasmic staining when used in immunohistochemistry. For staining of formalin-fixed paraffin sections, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesPalovuori, R., and Eskelinen, S. 2000 Eur. J. Cell Biol. 79, 961.
      Bakolitsa, C., et al. 1999 Cell 99, 603.
      Rudiger, M., et al. 1998 Bioessays 20, 733
      Tidball, J.G., et al. 1999 J. Biol. Chem. 274, 33155
      Goldmann, W.H., et al. 1996 J. Muscle Res. Cell Motil. 17, 1.
      Asijee, G.M., et al. 1990 Eur. J. Biochemistry 189, 131
      Otto, J.J. 1990 Cell Motil. Cytoskeleton 16, 1.