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CP06 Anti-α-Tubulin Mouse mAb (DM1A)

This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.

Anti-α-Tubulin Mouse mAb (DM1A) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP06
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ch, Gr, H, M, RMMonoclonal Antibody

Pricing & Availability

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CP06-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.
      Catalogue NumberCP06
      Brand Family Calbiochem®
      References
      ReferencesBlose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
      Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
      Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
      Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
      Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Negative controltrp E (Ab-1)
      Positive controlAny cell line
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)
      Immunofluorescence (5 µg/ml)
      Application CommentsTubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogennative chick brain microtubules
      ImmunogenChicken
      CloneDM1A
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Chicken
      • Gerbil
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP06-100UG 04055977220933

      Documentation

      Anti-α-Tubulin Mouse mAb (DM1A) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-α-Tubulin Mouse mAb (DM1A) Certificates of Analysis

      TitleLot Number
      CP06

      References

      Reference overview
      Blose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
      Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
      Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
      Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
      Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.

      Citations

      Title
    • Saumen Pal, et al. (2006) An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis. Journal of Cell Biology 174, 1047-1058.
    • Zhonghua Gao, Yufang Shao and Xuejun Jiang. (2005) Essential roles of the Bcl-2 family of proteins in caspase-2-induced apoptosis. Journal of Biological Chemistry 280, 38271-38275.
    • Annie Huang, et al. (2005) Identification of a novel c-myc protein interactor, JPO2, with transforming activity in medulloblastoma cells. Cancer Research 65, 5607-5619.
    • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision30-November-2007 JSW
      ApplicationImmunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)
      Immunofluorescence (5 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.
      BackgroundCytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape, and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
      HostMouse
      Immunogen speciesChicken
      Immunogennative chick brain microtubules
      CloneDM1A
      IsotypeIgG₁
      Specieschicken, gerbil, human, mouse, rat
      Positive controlAny cell line
      Negative controltrp E (Ab-1)
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsTubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesBlose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
      Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
      Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
      Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
      Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
      Citation
    • Saumen Pal, et al. (2006) An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis. Journal of Cell Biology 174, 1047-1058.
    • Zhonghua Gao, Yufang Shao and Xuejun Jiang. (2005) Essential roles of the Bcl-2 family of proteins in caspase-2-induced apoptosis. Journal of Biological Chemistry 280, 38271-38275.
    • Annie Huang, et al. (2005) Identification of a novel c-myc protein interactor, JPO2, with transforming activity in medulloblastoma cells. Cancer Research 65, 5607-5619.
    • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
      		•
      Application referencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.