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GR08L Anti-Transferrin Receptor (Ab-1) Mouse mAb (T56/14)

Overview

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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GR08L-100UG
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      Description
      OverviewRecognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor in the plasma membrane in proliferating cells.
      Catalogue NumberGR08L
      Brand Family Calbiochem®
      SynonymsAnti-TR, Anti-CD71
      References
      ReferencesSchneider, C., et al., 1982. J. Biol. Chem. 257, 8516.
      Trowbridge, I.S. and Lopez, F., 1982. Proc. Natl. Acad. Sci. USA, 1175.
      Aisen, P. and Listowsky, I., 1980. Ann. Rev. Biochem. 49, 357.
      Omary, M.B., et al. 1980. Nature 286, 888.
      Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlMost cell lines or placental tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Trowbridge, I.S. and Omary, M.B., 1981. Proc. Natl. Acad. Sci. USA 3039.
      Key Applications Flow Cytometry
      Immunofluorescence
      Immunoprecipitation
      Not Western Blot
      Application NotesFlow Cytometry (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Immunoblotting (not recommended)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenK562 cells
      ImmunogenHuman
      CloneT56/14
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
      Global Trade Item Number
      Catalogue Number GTIN
      GR08L-100UG 04055977228199