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05-1531 Anti-TATA-box-binding protein Antibody, clone SL30-3-563

Use Anti-TATA-box-binding protein Antibody, clone SL30-3-563 (mouse monoclonal antibody) validated in WB to detect TATA-box-binding protein also known as TATA box binding protein, Transcription initia

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: GTF2D, TFIID, TBP, GTF2D, SCA17, TF2D UniProt Number: P20226
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05-1531
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1531
      DescriptionAnti-TATA-box-binding protein Antibody, clone SL30-3-563
      Alternate Names
      • TATA box binding protein
      • Transcription initiation factor TFIID TBP subunit
      • TATA-binding factor
      • TATA sequence-binding protein
      • TATA-box binding protein N-terminal domain
      • TATA-box factor
      Background InformationThe TATA-box-binding protein (TBP) is a critical, eukaryotic transcription factor. TBP a part of the RNA polymerase II eukaryotic transcription complex and is required for the transcriptional initiation of RNA polymerases. TBP is considered to be a limiting factor for certain RNA promoters. Expression is ubiquitous, however the highest expression has been observed in testis and ovary. Mutations in TBP result in spinocerebellar ataxia type 17(SCA17), a cerebellar disorder classified as autosomal dominant cerebellar ataxia and characterized by atrophy to the cerebellar and cerebral regions, extrapyramidal signs, and dementia.
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-TATA-box-binding protein Antibody, clone SL30-3-563 (mouse monoclonal antibody) validated in WB to detect TATA-box-binding protein also known as TATA box binding protein, Transcription initiation factor TFIID TBP subunit.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in U2OS cells. (Denisso, S., et al. (2007). The EMBO Journal. 26:944–954.)
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human TATA-box-binding protein at the N-terminus.
      EpitopeN-terminus
      CloneSL30-3-563
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes TATA-box-binding protein at the N-terminus.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with mouse and rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryInitiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes TBP, the TATA-binding protein. A distinctive feature of TBP is a long string of glutamines in the N-terminus. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. The number of CAG repeats encoding the polyglutamine tract is usually 32-39, and expansion of the number of repeats increases the length of the polyglutamine string and is associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • GTF2D
      • TFIID
      • TBP
      • GTF2D
      • SCA17
      • TF2D
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.

      SUBUNIT STRUCTURE: Binds DNA as monomer. Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B TAF1C and TAF1D. Association of TBP to form either TFIID or SL1/TIF-IB appears to be mutually exclusive. Interacts with TAF1A, TAF1B and TAF1C. Interacts with TFIIB, NCOA6, DRAP1, DR1 and ELF3. Interacts with SPIB, SNAPC1, SNAPC2 and SNAPC4. Interacts with UTF1. Interacts with BRF2. Interacts with UBTF. Interacts with GPBP1 By similarity. Interacts with HIV-1 Tat.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Widely expressed, with levels highest in the testis and ovary.

      POLYMORPHISM: The poly-Gln region of TBP is highly polymorphic (25 to 42 repeats) in normal individuals and is expanded to about 47-63 repeats in spinocerebellar ataxia 17 (SCA17) patients.

      INVOLVEMENT IN DISEASE: Defects in TBP are the cause of spinocerebellar ataxia type 17 (SCA17) [MIM:607136]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA17 is an autosomal dominant cerebellar ataxia (ADCA) characterized by widespread cerebral and cerebellar atrophy, dementia and extrapyramidal signs. The molecular defect in SCA17 is the expansion of a CAG repeat in the coding region of TBP. Longer expansions result in earlier onset and more severe clinical manifestations of the disease.

      SEQUENCE SIMILARITIES: Belongs to the TBP family.
      Molecular Weight ~38 kDa observed.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected TATA-box-binding protein on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      05-1531 04053252640940