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MABN1832 Anti-SK2 (KCa2.2) Antibody, clone K78/29

Anti-SK2 (KCa2.2) Antibody, clone K78/29 is an antibody against SK2 for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-SK2 (KCa2.2) Antibody, clone K78/29 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: Kcnn2 UniProt Number: P70604
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MABN1832
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, MWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1832
      DescriptionAnti-SK2 (KCa2.2) Antibody, clone K78/29
      Alternate Names
      • Small conductance calcium-activated potassium channel protein 2
      • SK2
      • SKCa 2
      • SKCa2
      • KCa2.2
      Background InformationSmall conductance calcium-activated potassium channel protein 2 (UniProt P70604; also known as SK2, SKCa 2, SKCa2, KCa2.2) is encoded by the Kcnn2 gene (Gene ID 54262) in rat species. Small conductance calcium-activated potassium channels mediate cell membrane repolarization following intracellular Ca2+ increase induced by various stimuli, thereby restoring an excited cell back to its resting state. SK2 is involved in afterhyperpolarization (AHP) that follows action potentials in vertebrate neurons. SK2 regulates neuronal excitability by contributing to the slow component of synaptic AHP. Ca2+ activates SK2 through the Ca2+ sensor calmodulin (CaM), which can bind up to four Ca2+ ions. The dynamic coupling between Ca2+ binding to CaM, CaM binding to SK, and the open probability of SK are all essential components of the SK2 activity.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SK2 (KCa2.2) Antibody, clone K78/29 is an antibody against SK2 for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: An 1:50 dilution from a representative lot detected SK2 (KCa2.2) immunoreactivity in human cerebellum, human cerebral cortex, mouse brain, mouse skeletal muscle, rat kidney and rat testis tissue sections.
      Western Blotting Analysis: A representative lot detected the transiently expressed rat SK2 (KCa2.2), but not SK1 (KCa2.1), SK3 (KCa2.3), SK4 (KCa3.1, KCa4), or Kv2.1 constructs in transfected COS-7 cells (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
      Immunohistochemistry Analysis: A representative lot detected SK2 (KCa2.2) immunoreactivity in adult rat brain hippocampus sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
      Biological Information
      ImmunogenLinear peptide corresponding to a C-terminal cytoplasmic domain sequence of rat SK2 (KCa2.2).
      EpitopeC-terminal cytomplasmic region.
      CloneK78/29
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone K78/29 detects endogenous KCa2.2 in rat brain membrane extracts, as well as the transiently expressed rat SK2 (KCa2.2), but not SK1 (KCa2.1), SK3 (KCa2.3), SK4 (KCa3.1, KCa4), or Kv2.1 constructs in transfected COS-7 cells. Exprected to react with both spliced isoforms of human KCa2.2 reported by UniProt (Q9H2S1).
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Kcnn2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~45 kDa observed. 63.85 kDa (rat), 63.76 kDa (human isoform 1), 26.34 kDa (human isoform 2), 91.60 kDa (mouse) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected SK2 (KCa2.2) in 10 µg rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1832 04055977329544