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MAB353B Anti-Nestin Antibody, clone rat-401, Biotin Conjugate

Detect Nestin using this mouse monoclonal antibody, Anti-Nestin Antibody, clone rat-401, Biotin Conjugate validated for use in ICC & IHC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research, Neuroscience Research Sub-Category: Developmental Neuroscience, Neural Stem Cells Gene Symbol: NES, Nbla00170, FLJ21841 UniProt Number: P21263
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MAB353B
50 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RICC, IHCMBiotinMonoclonal Antibody
      Description
      Catalogue NumberMAB353B
      DescriptionAnti-Nestin Antibody, clone rat-401, Biotin Conjugate
      Alternate Names
      • rat401
      • NES
      • Nbla00170
      • FLJ21841
      Background InformationNestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.
      References
      Product Information
      FormatBiotin
      Control
      • Mouse cortical neural stem cells (SCR029)
      PresentationPurified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.
      Quality LevelMQ100
      Applications
      ApplicationDetect Nestin using this mouse monoclonal antibody, Anti-Nestin Antibody, clone rat-401, Biotin Conjugate validated for use in ICC & IHC.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesEvaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022).
      Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).
      Evaluated by Immunocytochemistry in ReNcell VM human neural progenitor cells (SCC008).
      Immunocytochemsitry Analysis: A 1:200 dilution of this antibody did not detect Nestin in ReNcell VM human neural progenitor cells (SCC008). Evaluated by Immunohistochemistry in rat hindbrain brain tissue.
      Immunohistochemsitry Analysis: A 1:50 dilution of this antibody detected Nestin in rat hindbrain brain tissue.
      Biological Information
      ImmunogenEmbryonic rat spinal cord
      Clonerat-401
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteDemonstrated to react with Mouse and Rat. Antibody is not reactive with human Nestin protein.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NES
      • Nbla00170
      • FLJ21841
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight200-220 kDa calculated
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:200 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB353B 04053252917622

      Documentation

      Anti-Nestin Antibody, clone rat-401, Biotin Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nestin Antibody, clone rat-401, Biotin Conjugate Certificates of Analysis

      TitleLot Number
      Anti-Nestin, clone rat-401, Biotin -2781578 2781578
      Anti-Nestin, clone rat-401, Biotin Conjugate - 3784482 3784482
      Anti-Nestin, clone rat-401, Biotin Conjugate - Q2246036 Q2246036
      Anti-Nestin, clone rat-401, Biotin Conjugate -2575774 2575774
      Anti-Nestin, clone rat-401, Biotin Conjugate -2636736 2636736

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies