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MAB365 Anti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG

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MAB365
50 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RICC, IHC, IP, FUNC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB365
      Replaces04-1111
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG
      Alternate Names
      • CD271 antigen
      • Low affinity neurotrophin receptor p75NTR
      • Low-affinity nerve growth factor receptor
      • NGF receptor
      • low affinity nerve growth factor receptor
      • nerve growth factor receptor
      • nerve growth factor receptor (TNFR superfamily, member 16)
      • p75 ICD
      Background InformationThe low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells.
      Product Information
      FormatPurified
      Control
      • Western Blot: NGF-differentiated PC12 cell lysate.
        Immunohistochemistry: Rat brain tissue.
      PresentationMouse monoclonal IgG1 in 0.02M Phosphate buffer, pH7.6, 0.25M NaCl with 0.1% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nerve Growth Factor Receptor Antibody, extracellular, clone 192-IgG is an antibody against Nerve Growth Factor Receptor for use in IC, IH, IP, FUNC & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Affects Function
      • Western Blotting
      Application NotesWestern Blot:
      1-5 µg/mL (Non-reducing conditions only) was used on a previous lot.

      Inhitibition Assay:
      Inhibition on NGF binding {Chandler, 1984}.

      Immunoprecipitation:
      5 µg/mL, 0.5% triton X-100 buffer; PC12 positive control was used on a previous lot.

      Immunohistochemistry:
      1-5 µg/mL on rat spinal chord, of a previous lot was used. Other fixatives and methods can also be used.
      Biological Information
      Immunogenn-octoglucoside stabilized proteins containing p75 receptor, which were isolated from plasma membranes of PC-12 cells (Chandler et al., 1984).
      EpitopeExtracellular
      Clone192-IgG
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificitySpecific for the rat low affinity Nerve Growth Factor-Receptor (p75), does not react with human or mouse p75.
      IsotypeIgG1
      Species Reactivity
      • Rat
      Species Reactivity NoteReacts with rat p75 protein.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
      Gene Symbol
      • NGFR
      • p75(NTR)
      • TNFRSF16
      • Gp80-LNGFR
      • CD271
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
      SIZE: 427 amino acids; 45183 Da
      SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9.
      PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues.
      SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.
      Molecular Weight75 kDa
      Product Usage Statements
      Quality AssuranceImmunohistochemistry:
      1-5 µg/mL on rat , of a previous lot, (spinal chord, see protocol below); other fixatives and methods can also be used.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size50 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB365 04053252727252