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IM09L Anti-MMP-9 (Ab-1) Mouse mAb (6-6B)

This Anti-MMP-9 (Ab-1) Mouse mAb (6-6B) is validated for use in Immunoblotting, Immunoprecipitation, Neutralization Studies, Paraffin Sections for the detection of MMP-9 (Ab-1).

Anti-MMP-9 (Ab-1) Mouse mAb (6-6B) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase, Anti-Gelatinase B

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IM09L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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IM09L-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~92 kDa latent and the ~83 kDa active forms of MMP-9 under non-reducing conditions, but only the latent form under reducing conditions. Inhibits the enzymatic activity of MMP-9. For paraffin sections, use Cat. No. IM37L.
      Catalogue NumberIM09L
      Brand Family Calbiochem®
      SynonymsAnti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase, Anti-Gelatinase B
      References
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol. 2, 861.
      Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
      Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349.
      Stetler-Stevenson, W.G., et al. 1993. FASEB. 7, 1434.
      Zucker, S., et al. 1993. Cancer Res. 53, 140.
      Woessner, J.F. 1991. FASEB. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
      Product Information
      DeclarationNot available for sale in Japan.
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlMMP-9 protein (Cat. Nos. PF024 or PF038)
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci. 732, 469. Immunoblotting, Neutralization Studies Ramos-DeSimone, N., et al. 1993. Hybridoma. 12, 349.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Not Paraffin Sections
      Neutralization Studies
      Application NotesImmunoblotting (2 µg/ml)
      Immunoprecipitation (see appplication references)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (not recommended)
      Application CommentsInhibits MMP-9 enzymatic activity. For staining paraffin sections, use Anti-MMP-9 (626-644) (Ab-3) Mouse mAb (56-2A4) (Cat. No. IM37L). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenMMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells
      ImmunogenHuman
      Clone6-6B
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsStore at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Resuspend the antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Freezing of aliquots is best for long-term storage of reconstituted product; repetitive freezing and thawing should be avoided.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM09L-100UG 04055977228151

      Documentation

      Anti-MMP-9 (Ab-1) Mouse mAb (6-6B) Certificates of Analysis

      TitleLot Number
      IM09L

      References

      Reference overview
      Cottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol. 2, 861.
      Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
      Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349.
      Stetler-Stevenson, W.G., et al. 1993. FASEB. 7, 1434.
      Zucker, S., et al. 1993. Cancer Res. 53, 140.
      Woessner, J.F. 1991. FASEB. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase, Anti-Gelatinase B
      ApplicationImmunoblotting (2 µg/ml)
      Immunoprecipitation (see appplication references)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~92 kDa latent and the ~83 kDa active forms of human MMP-9 under non-reducing conditions, but only the latent form under reducing conditions.
      BackgroundMatrix metalloproteinases (MMP's) are a family of enzymes that are responsible for the degradation of extracellular matrix components such as collagen, laminin and proteoglycans. In addition to sequence homology, all MMP's share the following characteristics: the catalytic mechanism is dependent upon a zinc ion at the active center, they cleave one or more extracellular matrix components, they are secreted as zymogens which are activated by removal of an ~10 kDa segment from the N terminus and they are inhibited by tissue inhibitor of metalloproteinases (TIMP). These enzymes are involved in normal physiological processes such as embryogenesis and tissue remodeling and may play an important role in arthritis, periodontitis, and metastasis. MMP-9 (Gelatinase B, 92 kDa gelatinase/type IV collagenase) is secreted as a 92 kDa zymogen which is proteolytically processed to the 83 kDa active form; a 68 kDa has also been detected. MMP-9, along with its most closely related member of the MMP family, MMP-2, show substrate specificity toward type IV and V collagens, gelatin and elastin. Numerous studies have shown a correlation between collagenase expression and metastatic potential. Elevated levels of MMP-9 in plasma suggest that it may be a useful marker for the diagnosis or prognosis of cancer in general.
      HostMouse
      Immunogen speciesHuman
      ImmunogenMMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells
      Clone6-6B
      IsotypeIgG₁
      Specieshuman
      Positive controlMMP-9 protein (Cat. Nos. PF024 or PF038)
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsInhibits MMP-9 enzymatic activity. For staining paraffin sections, use Anti-MMP-9 (626-644) (Ab-3) Mouse mAb (56-2A4) (Cat. No. IM37L). Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsStore at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Resuspend the antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Freezing of aliquots is best for long-term storage of reconstituted product; repetitive freezing and thawing should be avoided.
      Toxicity Standard Handling
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol. 2, 861.
      Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
      Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349.
      Stetler-Stevenson, W.G., et al. 1993. FASEB. 7, 1434.
      Zucker, S., et al. 1993. Cancer Res. 53, 140.
      Woessner, J.F. 1991. FASEB. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
      Application referencesImmunoprecipitation Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci. 732, 469. Immunoblotting, Neutralization Studies Ramos-DeSimone, N., et al. 1993. Hybridoma. 12, 349.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies