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MABF2304F-25UL Anti-IL-9 receptor Antibody, clone RZ-66 Antibody, FITC Conjugate

Anti-IL-9 receptor, clone RZ-66, FITC Conjugate, Cat. No. MABF2304F, is a rat monoclonal antibody that detects Interleukin-9 receptor and is tested for use in Flow Cytometry.

MABF2304F-25UL
25 μL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2304F-25UL
      DescriptionAnti-IL-9 receptor Antibody, clone RZ-66 Antibody, FITC Conjugate
      Alternate Names
      • Interleukin-9 receptor
      • IL-9R
      • CD129
      Background InformationInterleukin-9 receptor (UniProt: Q01114; also known as IL-9 receptor, IL-9R, CD129) is encoded by the Il9r gene (Gene ID: 16199) in murine species. IL-9R is a single-pass type I membrane protein that is synthesized with a signal peptide (aa 1-37), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 38-270), a transmembrane domain (aa 271-291), and a cytoplasmic domain (aa 291-468). It contains a WSXWS motif (aa 244-248) that is essential for proper protein folding and cell surface receptor binding. It also contains a Box-1 motif (aa 301-309) that is required for JAK interaction and/or activation. IL-9R is composed of the IL-9R a-chain and common g-chain. IL-9R is expressed on mast cells, activated T cells, ILC2 cells, and basophils. It is also selectively induced on B memory cells after primary immunization and this expression is induced by stimulation with CD40. IL-9R expression on B memory cells is shown to be essential for a normal recall antibody response in murine models. Signaling by IL-9R is shown to result in the activation of major signaling pathways involving STAT1, STAT-3, and STAT-5, MAPK, and PI3K. IL-9R signaling is shown to be pro-inflammatory and it promotes allergic inflammation and airway remodeling in lungs. It is also important in cell growth and differentiation of T cells, B cells, mast cells, hematopoietic progenitors, epithelial cells, and airway smooth muscle cells. (Ref.: Takatsuka, S., et al. (2018). Nat. Immunol. 19 (9); 1025-1034).
      References
      Product Information
      FormatFITC
      PresentationPurified rat monoclonal antibody IgG1 in buffer containing PBS 0.01 M, sodium azide, 0.1%, pH7.1 and 15 mg/mL BSA with 0.01% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-IL-9 receptor, clone RZ-66, FITC Conjugate, Cat. No. MABF2304F, is a rat monoclonal antibody that detects Interleukin-9 receptor and is tested for use in Flow Cytometry.
      Key Applications
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 3 µg/mL from a representative lot detected IL-9 receptor in B300-19 cells.

      Flow Cytometry Analysis: A representative lot detected IL-9 receptor in Flow Cytometry applications (Takatsuka, S., et. al. (2018). Nat Immunol.19(9):1025-1034).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenpCADEST1 vector carrying a gene encoding for murine IL-9R GroEL fusion protein.
      EpitopeUnknown
      CloneRZ-66
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone RZ-66 is a rat monoclonal antibody that detects murine IL-9 receptor.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Il9r
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight52.26 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in RAW264.7 cells.

      Flow Cytometry Analysis: 2 µg of this antibody detected IL-9 receptor in one million RAW264.7 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2304F-25UL 04065265324298