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Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation.
More>>Anti-Histone H3, Acetylated (1-20), rabbit polyclonal, recognizes the ~17 kDa acetylated histone H3 protein. It is validated for use in Western blotting, immunocytochemistry & immunoprecipitation. Less<<
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Description
Overview
Recognizes the ~17 kDa acetylated histone H3 protein.
Catalogue Number
382158
Brand Family
Calbiochem®
Application Data
Detection of human acetylated histone H3 by immunoblotting. Samples: Acid-extract cells left untreated (lane 1) and sodium butyrate treated (lane 2). Primary antibody: Anti-Histone H3, Acetylated (1-20) Rabbit pAb (Cat. No. 382158) (0.01 µg/ml). Detection: chemiluminescence.
References
References
Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349. Braunstein, M., et al. 1993. Genes Devel.7, 592. Perry, C.A., et al. 1993. Biochemistry32, 13605. Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
Immunoblotting (10-500 ng/ml, chemiluminescence) Immunocytochemistry (5 µg/ml) Immunoprecipitation (see comments)
Application Comments
For immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogen
a diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH
Immunogen
Human
Host
Rabbit
Isotype
IgG
Species Reactivity
Human
Tetrahymena
Antibody Type
Polyclonal Antibody
Concentration Label
Please refer to vial label for lot-specific concentration
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Anti-Histone H3, Acetylated (1-20) Rabbit pAb Certificates of Analysis
Title
Lot Number
382158
References
Reference overview
Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349. Braunstein, M., et al. 1993. Genes Devel.7, 592. Perry, C.A., et al. 1993. Biochemistry32, 13605. Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
02-August-2007 RFH
Application
Immunoblotting (10-500 ng/ml, chemiluminescence) Immunocytochemistry (5 µg/ml) Immunoprecipitation (see comments)
Application Data
Detection of human acetylated histone H3 by immunoblotting. Samples: Acid-extract cells left untreated (lane 1) and sodium butyrate treated (lane 2). Primary antibody: Anti-Histone H3, Acetylated (1-20) Rabbit pAb (Cat. No. 382158) (0.01 µg/ml). Detection: chemiluminescence.
Description
Protein A purified rabbit polyclonal antibody. Recognizes the ~17 kDa acetylated histone H3 protein.
Background
The acetylation of core histones regulates gene expression through alterations in the chromatin structure. Lysine acetylation in the amino-terminal domains of histones H3 and H4 can alter the topology of the DNA in chromatin and probably influence its higher-order folding.
Host
Rabbit
Immunogen species
Human
Immunogen
a diacetylated synthetic peptide [ARTKQTARAcKSTGGAcKAPRKQL(C)] corresponding to amino acids 1-20 of human histone H3, conjugated to KLH
Isotype
IgG
Species
Tetrahymena, human
Positive control
HeLa cells
Form
Liquid
Formulation
In 150 mM NaCl, 100 mM Tris-glycine, pH 7.4.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
For immunocytochemistry, HeLa cells were fixed with 95% ethanol/5% acetic acid and treated with 5 mM sodium butyrate, an inhibitor of histone deacetylases, for 24 h. 10-500 ng/ml of antibody detected acetylated histone H3 in acid-extracted proteins from HeLa cells. Using sodium butyrate, an inhibitor of histone deacetylases, enhances detection of acetylated histone H3. Higher concentrations of antibody also detected unacetylated histone H3. Preliminary evidence from an independent library suggests that phosphorylation of Ser10 on histone H3 may disrupt epitope recognition by this antibody. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Toxicity
Standard Handling
References
Braunstein, M., et al. 1996. Mol. Cell. Biol.16, 4349. Braunstein, M., et al. 1993. Genes Devel.7, 592. Perry, C.A., et al. 1993. Biochemistry32, 13605. Lin, R., et al. 1989. J. Cell. Biol.108, 1577.
