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MAB397A4 Anti-GluR2 Antibody, clone 6C4, Alexa Fluor® 488 Conjugate

This Anti-GluR2 Antibody, clone 6C4, Alexa Fluor 488 Conjugate is validated for use in IH, IC for the detection of GluR2.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: Gria2, GLUR2, GLURB, GluR-2, GluR-B UniProt Number: P19491
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MAB397A4
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RIHC, ICCMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue NumberMAB397A4
      DescriptionAnti-GluR2 Antibody, clone 6C4, Alexa Fluor® 488 Conjugate
      Alternate Names
      • Glutamate receptor 2
      • GluR-2
      • AMPA-selective glutamate receptor 2
      • GluR-B
      • GluR-K2
      • Glutamate receptor ionotropic, AMPA 2
      Background InformationGlutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this positionis occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks CA2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Adult mouse brain tissue
      PresentationPurified mouse monoclonal IgG2a conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-GluR2 Antibody, clone 6C4, Alexa Fluor 488 Conjugate is validated for use in IH, IC for the detection of GluR2.
      Key Applications
      • Immunohistochemistry
      • Immunocytochemistry
      Application NotesImmunocytochemistry analysis: A 1:100 dilution from a representative lot detected GluR2 in rat E18 primary cortex cells.
      Biological Information
      ImmunogenRecombinant fusion protein TrpE-GluR2
      EpitopeExtracellular domain
      Clone6C4
      HostMouse
      SpecificityRecognizes the large N-terminal extracellular domain of Glutamate Receptor 2 (GluR2). No cross-reactivity observed with other AMPA/Kainate GluR subunits.
      IsotypeIgG2a
      Species Reactivity
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene.
      Gene Symbol
      • Gria2
      • GLUR2
      • GLURB
      • GluR-2
      • GluR-B
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.

      SIZE: 883 amino acids; 98,688 Da

      SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. May interact with MPP4. Forms a ternary complex with GRIP1 and CSPG4 (By similarity). Interacts with CACNG2, PRKCABP, GRIP1 and GRIP2. Interacts with NSF via its C-terminus. Part of a complex containing GRIA2, NSF and NAPA and/or NAPB.

      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Note= Interaction with CACNG2 promotes cell surface expression.

      TISSUE SPECIFICITY: Detected in forebrain. Detected in dendrites of neuronal cells.

      SIMILARITY: Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
      Molecular WeightThe unconjugated parent antibody (Cat. No. MAB397) has an observed molecular weight at ~102 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in adult mouse brain tissue.

      Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected GluR2 in adult mouse brain tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB397A4 04053252420870

      Documentation

      Anti-GluR2 Antibody, clone 6C4, Alexa Fluor® 488 Conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GluR2 Antibody, clone 6C4, Alexa Fluor® 488 Conjugate Certificates of Analysis

      TitleLot Number
      Anti-GluR2, clone 6C4, Alexa Fluor#174; 488 Conjugate - 2029164 2029164
      Anti-GluR2, clone 6C4, Alexa Fluor#174; 488 Conjugate - 2161206 2161206
      Anti-GluR2, clone 6C4, Alexa Fluor#174; 488 Conjugate - 2266773 2266773
      Anti-GluR2, clone 6C4, Alexa Fluor#174; 488 Conjugate - NRG1922537 NRG1922537
      Anti-GluR2, clone 6C4, Alexa Fluor® -2760517 2760517
      Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate 2956761
      Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate 2794938
      Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate - 2377087 2377087
      Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate - 2315733 2315733
      Anti-GluR2, clone 6C4, Alexa Fluor® 488 Conjugate - 2361550 2361550
      Show more

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies