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MABE1985-100UG Anti-Gemin5 Antibody, clone 1E12

Anti-Gemin5, clone 1E12 , Cat. No. MABE1985, is a mouse monoclonal antibody that detects Gemin5 and is tested for use in Immunocytochemistry and Western Blotting.

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MABE1985-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABE1985-100UG
      DescriptionAnti-Gemin5 Antibody, clone 1E12
      Alternate Names
      • Gem-associated protein 5
      Background InformationGem-associated protein 5 (UniProt: Q8TEQ6; also known as Gemin5) is encoded by the GEMIN5 gene (Gene ID: 25929) in human. Survival of motor neurons (SMN) protein is known to form a stable complex with a group of proteins named gemins. The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs) and thereby plays an important role in the splicing of cellular pre-mRNAs. Gemin5, a highly conserved, multi-functional protein, is a component of the core SMN complex and is found in the nucleoplasm and nuclear bodies known as Gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. It is also abundantly present in the cytoplasm. It plays a role in capturing snRNA into SMN complexes in the cytoplasm for transport into the nucleus. However, in the nucleus it is easily detached from the SMN-Gemin complex. Gemin5 is reported to physically bind to snRNA via a specific AU5-6 sequence located within the highly conserved Sm site and is flanked by a short stem loop that assists in delivery to the SMN complex. The specific snRNP code helps Gemin5 in distinguishing these snRNAs from other forms of cellular RNAs. Gemin5 contains 13 WD repeat domains that mediate its binding to U1 and U4 snRNA. WD domains are also reported to mediate binding to the 7-methylguanosine cap that is found on mRNA and snRNA molecules. Mutations in GEMIN5 gene are known to cause a distinct neurological cerebellar ataxia syndrome, through altered snRNP complex assembly. (Ref.: Kour, S., et al. (2021). Nat. Commun. 12; Article 2558; Hao, L., et al. (2007). BMC Cell Biol. 8:28).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Gemin5, clone 1E12 , Cat. No. MABE1985, is a mouse monoclonal antibody that detects Gemin5 and is tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesTested Applications

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in HeLa cell lysate.

      Immunocytochemistry Analysis: A representative lot detected Gemin5 in Immunocytochemistry application (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28; Schilling, M., et al. (2021). Cell Rep. 35(12); 109277).

      Western Blotting Analysis: A representative lot detected Gemin5 in Western Blotting applications (Hao, L.T., et al. (2007). BMC Cell Biol. 8; 28).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 292 amino acids form the C-terminal region of human Gem-associated protein 5 (Gemin5).
      EpitopeC-terminal
      Clone1E12
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1E12 is a mouse monoclonal antibody that detects Gem-associated protein 5 (Gemin5). It targets an epitope within the C-terminal region.
      IsotypeIgG1
      Species Reactivity
      • Monkey
      • Human
      Species Reactivity NoteHuman, Monkey. Predicted to react with Porcine based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GEMIN5
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~170 kDa observed; 168.59 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in COS-7 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Gemin5 in COS-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1985-100UG 04065268529003