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MABN875 Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7

This Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, ELISA for the detection of GABAA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: GABRG2 UniProt Number: P18507
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MABN875
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, BWB, IH(P), IP, ELISAMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN875
      DescriptionAnti- GABAA Antibody γ2 Subunit, clone KC4-8A7
      Alternate Names
      • Gamma-aminobutyric acid receptor subunit gamma-2
      • GABA(A) receptor subunit gamma-2
      • GABAA γ2 Subunit
      Background InformationGamma-aminobutyric acid (GABA) receptor subunit gamma-2 (UniProt P18507; also known as GABA(A) receptor subunit gamma-2) is encoded by the GABRG2 (also known as CAE2, ECA2, GEFSP3) gene (Gene ID 2566) in human. The GABAA receptor (GABAAR) is an ionotropic receptor and ligand-gated ion channel. Upon GABA binding and activation, the GABAA receptor selectively conducts chloride ions through its pore, resulting in hyperpolarization of the neuron. The GABAAR is a pentameric complex composed of two alpha, two beta, and one gamma subunit arranged around a central pore. Each subunit contains four transmembrane domains with both the N- and C-terminus located extracellularly. In human, there exist six types of alpha subunits (encoded by GABRA1-6), three types of beta subunits (encoded by GABRB1-3), and three types of gamma subunits (encoded by GABRG1-3). The gamma 2 subunit is initially produced with an signal peptide sequence (a.a. 1-39), which is then cleaved off to yield the mature subunit (a.a. 40 – 467).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 is validated for use in Western Blotting, Immunohistochemistry (Paraffin), Immunoprecipitation, ELISA for the detection of GABAA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • ELISA
      Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected GABAA γ2 Subunit in 10 µg of mouse brain tissue lysate.
      Western Blotting Analysis: 1.0 µg/mL from a representative lot detected GABAA γ2 Subunit in 10 µg of human brain tissue lysate.
      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GABAA γ2 Subunit in human cerebral cortex tissue.
      Western Blotting Analysis: A representative lot detected human GABAA gamma2 subunit cytoplasmic domain GST fusion protein, as well as endogenous GABAA gamma2 subunit in purified bovine brain GABAA receptor complex (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated GABAA receptor complex from rat cerebral cortex membrane extracts, as well as purified bovine brain GABAA receptor complex as measured by ligand-binding activity associated with the immune complex (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311).
      ELISA Analysis: A representative lot detected human GABAA gamma2 cytoplasmic domain GST fusion protein, but not GST (Fernando, L.P., et al. (1995). J Neurochem. 64(3):1305-1311).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to the cytoplasmic domain of human GABAA γ2 Subunit.
      Epitopecytoplasmic domain
      CloneKC4-8A7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with all three spliced isoforms of GABA(A) receptor gamma 2 subunit (GABRG2), but not gamma 1 (GABRG1) or gamma 3 (GABRG3) subunit.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Bovine
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GABRG2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~45 kDa observed. Molecular weight bands were observed at ~45 kDa for both rat and mouse brain tissue lysates. However, for human brain tissue lysates, we detected a molecular weight at ~58 kDa, as predicted by Uniprot.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected GABAA γ2 Subunit in 10 µg of rat brain tissue lysate.
      Note: For Western blotting analysis, DO NOT BOIL samples prior to electrophoresis. After dissociating receptor complex with SDS sample buffer, remove non-solublized material by centrifugation.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN875 04055977277371

      Documentation

      Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti- GABAA Antibody γ2 Subunit, clone KC4-8A7 Certificates of Analysis

      TitleLot Number
      Anti- GABAA γ2 Subunit, -Q2602138 Q2602138
      Anti- GABAA γ2 Subunit, clone KC4-8A7 - 3310101 3310101

      References

      Reference overviewPub Med ID
      Monoclonal antibodies to the human gamma 2 subunit of the GABAA/benzodiazepine receptors.
      Fernando, LP; Khan, ZU; McKernan, RM; De Blas, AL
      Journal of neurochemistry  64  1305-11  1995

      Show Abstract       		7861163 7861163

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies