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MABF3145-25UG Anti-Envelope glycoprotein B Antibody, HCMV Antibody, clone 27-156

Anti-Envelope glycoprotein B, HCMV, clone 27-156, Cat. No. MABF3145, is a mouse monoclonal antibody that detects glycoprotein B and is tested in ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralizing, and Western Blotting.

MABF3145-25UG
25 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF3145-25UG
      DescriptionAnti-Envelope glycoprotein B Antibody, HCMV Antibody, clone 27-156
      Alternate Names
      • gB
      Background InformationEnvelope glycoprotein B (UniProt: P13201; also known as gB) is encoded by the gB (also known as UL55) gene (Gene ID: 3077424) in human cytomegalovirus (HHV-5). Human cytomegalovirus (HCMV), a member of the β-Herpesviridae family, is a major cause of morbidity and mortality in organ transplant recipients and patients with AIDS. Its envelope glycoprotein B is reported to be essential for the infectivity and is considered to be one of the most immunogenic virus-encoded proteins. It facilitates the entry of HCMV entry into cells by binding receptors and mediating membrane fusion. Glycoprotein B is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains a virion surface domain (aa 23-751), a transmembrane domain (aa 752-772), and an intra-virion domain (aa 773-907). The amino acid sequences 152-158 and 237-244 are critical for fusion and/or binding to host cell membrane. During virion morphogenesis, envelope glycoprotein B probably accumulates in the endosomes and trans-Golgi where secondary envelopment occurs. It is then transported to the cell surface from where it is endocytosed and is directed to the trans-Golgi network. The ectodomain of HCMV glycoprotein B forms rosette-like aggregates that are similar to rosettes formed by the EBV glycoprotein B ectodomain. Four exposed hydrophobic residues in its fusion loop are shown to be essential for rosette formation. A linear domain of glycoprotein B, designated at AD-1, represents a dominant antibody binding site on this protein and is also required for oligomerization of glycoprotein B. (Ref.: Sharma, S., et al. (2013). Virology. 435(2); 239-249; Britt, WJ., et al. (2005). J. Virol. 79(7); 4066-4079; Jean, F., et al. (2000). Proc. Natl. Acad. Sci. USA. 97(6); 2864-2869; Speckner, ., et al. (1999). J. Gen. Virol. 80(8); 2183-2191; Schoppel, K., et al. (1996). Virology. 216(1); 133-145).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Envelope glycoprotein B, HCMV, clone 27-156, Cat. No. MABF3145, is a mouse monoclonal antibody that detects glycoprotein B and is tested in ELISA, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Neutralizing, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Neutralizing
      • Western Blotting
      Application NotesTested applications

      Immunoprecipitation Analysis: A representative lot immunoprecipitated HCMV Envelope glycoprotein B in Immunoprecipitation applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Vanarsdall, A.L., et al. (2008). J Virol. 82(23);11837-50).

      Immunocytochemistry Analysis: A representative lot detected HCMV Envelope glycoprotein B in Immunocytochemistry applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); :2864-9; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Strive, T., et al. (2002). J Virol. 76(3); 1252-64).

      Neutralizing: A representative lot neutralized Envelope glycoprotein B of HCMV. (Schoppel, K., et al. (1996). Virology. 216(1);133-45; Kropff, B., et al. (1997). J Gen Virol. 78(Pt8); 1999-2007; Vanarsdall, A.L., et al. (2008). J Virol. 82(23); 11837-50).

      Immunofluorescence Analysis: A representative lot detected HCMV Envelope glycoprotein B in Immunofluorescence applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1);113-22; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); 2864-9; Meyer, G.A., et al. (2000). J Biol Chem. 275(6); 3857-66; Strive, T., et al. (2002). J Virol. 76(3); 1252-64; Vanarsdall, A.L., et al. (2008). J Virol. 82(23); 11837-50).

      ELISA Analysis: A representative lot detected HCMV Envelope glycoprotein B in ELISA applications (Sharma, S., et al. (2013). Virology. 435(2); 239-49).

      Western Blotting Analysis: A representative lot detected HCMV Envelope glycoprotein B in Western Blotting applications (Reschke, M., et al. (1995). J Gen Virol. 76(Pt1); 113-22; Schoppel, K., et al. (1996). Virology. 216(1); 133-45; Kropff, B., et al. (1997). J Gen Virol. 78(Pt8);1999-2007; Jean, F., et al. (2000). Proc Natl Acad Sci USA. 97(6); 2864-9; Sharma, S., et al. (2013). Virology. 435(2); 239-49; Khanh Le-Trilling, V. T., et al. (2020). Cell Rep. 30(7); 2248-2260).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenWhole Human cytomegalovirus (HCMV).
      EpitopeC-terminal half
      Clone27-156
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 27-156 is a mouse monoclonal antibody that detects the envelope glycoprotein B in Human cytomegalovirus (HCMV). It targets an epitope within the antigenic domain 1 (AD1) within the C-terminal half.
      IsotypeIgG2bκ
      Species Reactivity
      • Virus
      Species Reactivity NoteHuman cytomegalovirus (HCMV)
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • gB
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight120-140 kDa observed; 101.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant HCMV glycoprotein B.

      Western Blotting Analysis (WB): A 1:200,000 dilution of this antibody detected recombinant HCMV glycoprotein B.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF3145-25UG 04065269802372