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MABS1173 Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2

This Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 is validated for use in Western Blotting for the detection of E3 ubiquitin-protein ligase CBL-C.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: CBLC, CBL3, RNF57 UniProt Number: Q9ULV8
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MABS1173
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1173
      DescriptionAnti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2
      Alternate Names
      • E3 ubiquitin-protein ligase CBL-C
      • RING finger protein 57
      • SH3-binding protein CBL-3
      • SH3-binding protein CBL-C
      • Signal transduction protein CBL-C
      Background InformationE3 ubiquitin-protein ligase CBL-C (UniProt Q9ULV8; also known as RING finger protein 57, SH3-binding protein CBL-3, SH3-binding protein CBL-C, Signal transduction protein CBL-C) is encoded by the CBLC (also known as CBL3, RNF57) gene (Gene ID 23624) in human. CBLC belongs to the CBL family of E3 ubiquitin ligases, members of which also include CBL (a.k.a. c-CBL) and CBL-B. Similar to CBL and CBL-B, CBL-C binds SH3 (Src homology 3) domain-containing proto-oncogenic tyrosine kinases such as EGFR, Lyn, Crk, Src and Ret. CBL-C is recruited to EGFR upon EGF stimulation, leading to CBL-C phosphorylation by EGFR and subsequent downstream MAP kinase signalling. In addition to mediating oncoprotein Ret ubiquitination for proteasomal degradation, CBL-C is shown to form a complex with HIC5 (Hydrogen peroxide-inducible clone 5) and the heat shock protein HSP27 that are necessary for the ubiquitination of NOX4. Unlike the other CBL family members, CBL-C expression appears to be restricted to epithelial tissues. Transgenic expression of CBL-C in mouse mammary gland causes impairment of cell proliferation, suggesting that CBL-C might have a growth-inhibitory role. CBL-C contains an N-terminal Cbl-type phosphotyrosine-binding (Cbl-PTB) domain (a.a. 7-321) that mediates SH3 domain interaction and a C-terminal RET-interacting region (a.a. 351-474) harboring the catalytic ring finger domain (a.a. 351-390) that mediates interaction with E2 ubiquitin-conjugating enzyme. The PTB domain, also known as tyrosine kinase-binding (TKB) domain, is subdivided into a four-helix bundle or 4H domain (a.a. 7-145), a calcium-binding EF hand-like domain (a.a. 146-218), and a SH2-like domain (a.a. 219-321).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 is validated for use in Western Blotting for the detection of E3 ubiquitin-protein ligase CBL-C.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant fragment from the RET-interacting region of human CBL-C.
      EpitopeRET-interacting region.
      Clone10F4.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10F4.2 targets an epitope in the RET-interacting region present in both spliced isoforms of human CBL-C reported by UniProt (Q9ULV8).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CBLC
      • CBL3
      • RNF57
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~50 kDa observed. 52.46 kDa (isoform 1; Long) and 47.42 kDa (isoform 2; Short) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HT-29 human colon cancer cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected CBL-C in 10 µg of HT-29 human colon cancer cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1173 04055977352979

      Documentation

      Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-E3 ubiquitin-protein ligase CBL-C Antibody, clone 10F4.2 Certificates of Analysis

      TitleLot Number
      Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 - 3290410 3290410
      Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 - 3330357 3330357
      Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 - 3468666 3468666
      Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 - 3745497 3745497
      Anti-E3 ubiquitin-protein ligase CBL-C, clone 10F4.2 -Q2690249 Q2690249