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MAB3226 Anti-Cytokeratin 7 Antibody, clone RCK105

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MAB3226
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ca, H, Ht, M, Po, R, FeFC, WB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3226
      ReplacesMABT150
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytokeratin 7 Antibody, clone RCK105
      Background InformationKeratin, type II cytoskeletal 7 (UniProt: P08729; also known as Cytokeratin-7, CK-7, Keratin-7, K7, Sarcolectin, Type-II keratin Kb7) is encoded by the KRT7 (also known as SCL) gene (Gene ID: 3855) in human. Cytokeratins are the intermediate filament proteins of epithelia that display high degree of molecular diversity. They are heteropolymeric filaments formed by pairing of type I and type II keratins that are expressed in a highly specific patterns depending on the epithelial type and stage of cellular differentiation. They contain a central rod domain of about 310 amino acids with α-helical conformation bordered by non-helical head and tail domains of variable length. The head domain consists of subdomains V1 and H1. The central a-helical rod domain is composed of subdomains 1A, 1B, 2A, and 2B connected by the linkers L1, L12, and L2. The tail domain then consists of subdomains H2 and V2. They provide stability between epithelial cells and their attachment to basement membrane. Keratins also serve as tumor markers and epithelial differentiation markers. Multiple human epithelial keratins have been resolved with 2D-gels electrophoresis. These can be divided into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights that range from 40 to 55 kDa. The basic keratins have molecular weights that range from 56 to 70 kDa. Members of the acidic and basic subfamilies are reported to be present in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and the disease state. Keratin 7 is expressed in cultured epidermal, bronchial, and mesothelial cells. It is observed throughout the glandular cells in the junction between stomach and esophagus but is absent in esophagus. Its expression is also observed in a variety of carcinomas and in tumor cells of mammary and extramammary Paget disease. Keratin-7 expression can be used to distinguish ovarian and gastrointestinal carcinomas. (Ref.: Chu, P., et al. (2000). Modern Pathol. 13(9); 962-972; Kaba, A., et al. (1999). Biochimie. 81(7); 709-715).
      Product Information
      FormatPurified
      PresentationLiquid in buffer with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cytokeratin 7 Antibody, clone RCK105 is an antibody against Cytokeratin 7 for use in FC, WB, IC, IH.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern blot: 57-58kDa band in reduce westerns

      Immunohistochemistry on frozen tissue sections.

      Immunocytochemistry

      Flow cytometry

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCytokeratins from the human bladder carcinoma cell line T24.
      CloneRCK105
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityMAB3226 reacts with a subgroup of glandular epithelial and their tumors, as well as transitional epithelium and transitional carcinoma. It reacts with a 58 kDa protein corresponding to cytokeratin 7.
      IsotypeIgG1
      Species Reactivity
      • Canine
      • Human
      • Hamster
      • Mouse
      • Pig
      • Rat
      • Feline
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
      Gene Symbol
      • KRT7
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08729 # Plays a significant role in maintaining keratin filament organization in epithelia.
      SIZE: 469 amino acids; 51,386 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. Associates with KRT8.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Expressed predominantly in the epithelium and in carcinomas.
      PTM: Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
      SIMILARITY: SwissProt: P08729 Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3226 04053252660641