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MABE455 Anti-CHD-4 Antibody, clone 3F2/4

Detect Chd4 (Chromodomain-helicase-DNA-binding protein 4) using this mouse monoclonal antibody, Anti-CHD-4 Antibody, clone 3F2/4 validated for use in western blotting, IHC & Immunofluorescence.

Anti-CHD-4 Antibody, clone 3F2/4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: CHD4 UniProt Number: Q14839
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MABE455
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MChIP, WB, IHC, IFMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE455
      DescriptionAnti-CHD-4 Antibody, clone 3F2/4
      Alternate Names
      • Chromodomain-helicase-DNA-binding protein 4
      • CHD-4
      • ATP-dependent helicase CHD4
      • Mi-2 autoantigen 218 kDa protein
      • Mi2-beta
      Background InformationCHD-4, often referred to as Mi2-beta, is a central component of the nucleosome remodeling and histone deacetylase (NuRD) repressor complex. CHD-4 has been shown to interact with TRIM27, HDAC1, Histone deacetylase 2, MTA2, SATB1 and Ataxia telangiectasia and Rad3 related. This protein also interacts with KLF1. Sumoylation of KLF1 leads to transcriptional repression of CHD-4.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Chd4 (Chromodomain-helicase-DNA-binding protein 4) using this mouse monoclonal antibody, Anti-CHD-4 Antibody, clone 3F2/4 validated for use in western blotting, IHC & Immunofluorescence.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected CHD-4 in human breast carcinoma and skin tissues.

      Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.

      Immunofluorescence Analysis: A representative lot from an independent laboratory detected CHD-4 in mouse embryonic endothelial cells.
      Biological Information
      ImmunogenLinear peptide corresponding to human CHD-4.
      Clone3F2/4
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CHD4
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~220 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected CHD-4 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE455 04053252920936

      Documentation

      Anti-CHD-4 Antibody, clone 3F2/4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CHD-4 Antibody, clone 3F2/4 Certificates of Analysis

      TitleLot Number
      Anti-CHD-4, clone 3F2/4 - 3252104 3252104
      Anti-CHD-4, clone 3F2/4 - 3530664 3530664
      Anti-CHD-4, clone 3F2/4 - 3532474 3532474
      Anti-CHD-4, clone 3F2/4 - 3700366 3700366
      Anti-CHD-4, clone 3F2/4 - 3822329 3822329
      Anti-CHD-4, clone 3F2/4 - 4069762 4069762
      Anti-CHD-4, clone 3F2/4 - 4093866 4093866
      Anti-CHD-4, clone 3F2/4 - Q2239051 Q2239051
      Anti-CHD-4, clone 3F2/4 Monoclonal Antibody 2972041

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies