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MABN2418-25UL Anti-CELF4 Antibody, clone N446/80

Anti-CELF4, clone N446/80, Cat. No. MABN2418, is a highly specific mouse monoclonal antibody that targets CUGBP Elav-like family member 4 and has been tested for use in Western Blotting.

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MABN2418-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABN2418-25UL
      DescriptionAnti-CELF4 Antibody, clone N446/80
      Alternate Names
      • CUGBP Elav-like family member 4
      • Bruno-like protein 4
      • CUG-BP- and ETR-3-like factor 4
      • RNA-binding protein BRUNOL-4
      Background InformationCUGBP Elav-like family member 4 (UniProt: Q9BZC1; also known as CELF-4, Bruno-like protein 4, CUG-BP- and ETR-3-like factor 4, RNA-binding protein BRUNOL-4) is encoded by the CELF4 (also known as BRUNOL4) gene (Gene ID: 56853) in human. CELF-4 is a RNA-binding protein that is implicated in the regulation of pre-mRNA alternative splicing. It is ubiquitous in its distribution, but is strongly expressed in the cerebellum, hippocampus, amygdala, temporal and frontal cortex, and frontal lobes. It mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. It is shown to specifically activate exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. CELF-4 is reported to promote exclusion of both the smooth muscle and non-muscle exons in actinin pre-mRNAs. It binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. The N-terminal region (aa 1-298) is considered to be sufficient for its RNA-binding and MSE-dependent splicing activity. CELF-4 contains contain two N-terminal RNA recognition motif (RRM) domains (aa 54-135 and 152-232) and one C-terminal RRM domain (aa 404-479). Five isoforms of CELF-4 have been described that are produced by alternative splicing.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-CELF4, clone N446/80, Cat. No. MABN2418, is a highly specific mouse monoclonal antibody that targets CUGBP Elav-like family member 4 and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenA recombinant fragment corresponding to 86 amino acids from the N-terminal region of human CUGBP Elav-like family member 4 (CELF-4).
      EpitopeN-terminus
      CloneN446/80
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone N446/80 specifically detects CUGBP Elav-like family member 4 (CELF4). It targets an epitope with in 86 amino acids from the N-terminal region. It does not react with CELF6.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CELF4
      • BRUNOL4
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~46 kDa observed; 51.97 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human hippocampus tissue lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CELF4 in human hippocampus tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2418-25UL 04054839755286