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ABC962 Anti-CDC45

Detect CDC45 using this rabbit polyclonal Anti-CDC45 Antibody, Cat. No. ABC962, validated for use in Immunocytochemistry and Western Blotting.

Anti-CDC45 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: CDC45, CDC45L, CDC45L2, UNQ374/PRO710 UniProt Number: O75419
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ABC962
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC962
      DescriptionAnti-CDC45
      Alternate Names
      • Cell division control protein 45 homolog
      • PORC-PI-1
      Background InformationCell division control protein 45 homolog (UniProt O75419; also known as PORC-PI-1) is encoded by the CDC45 (also known as CDC45L, CDC45L2, UNQ374/PRO710) gene (Gene ID 8318) in human. Recruitment of replication initiation proteins at the G1/S transition enables origin DNA unwinding and loading of DNA polymerases. CDC45 is a replication initiation protein that plays an essential role in the establishment and progression of the replication fork. Cdc45 associates with the heterohexameric Mcm2-7 helicase and the GINS complex (go-ichi-nii-san, Japanese for 5-1-2-3, for the subunits Sld5, Psf1, Psf2 and Pfs3) to form the CMG (Cdc45-Mcm2-7-GINS) complex. CMG represents the eukaryotic replicative DNA helicase that unwinds origins of replication and drives the replication fork. CDC45 is also reported to interact with several other replication proteins, including DNA helicase B (DHB), Mcm10, Orc2, TopPB1, and DNA polymerases alpha, delta and epsilon.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect CDC45 using this rabbit polyclonal Anti-CDC45 Antibody, Cat. No. ABC962, validated for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CDC45 in L929 murine fibroblasts.
      Biological Information
      ImmunogenGST-tagged recombinant human CDC45 C-terminal fragment.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects CDC45 C-terminal region highly conserved among human, mouse, rat, and hamster species.
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse. Predicted to react with Hamster and Rat based on high sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CDC45
      • CDC45L
      • CDC45L2
      • UNQ374/PRO710
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~75 kDa observed. 65.57/60.25/68.77 kDa (human isoform 1/2/3), 65.38 kDa (mouse) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in L929 fibroblast lysate.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected CDC45 in 10 µg of L929 murine fibroblast lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC962 04054839052941