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ABE1858 Anti-Bora Antibody

Anti-Bora Antibody is an antibody against Bora for use in Western Blotting, Immunoprecipitation.

Anti-Bora Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: BORA, C13orf34 UniProt Number: Q6PGQ7
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ABE1858
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE1858
      DescriptionAnti-Bora Antibody
      Alternate Names
      • Protein aurora borealis
      • Bora
      • HsBora
      Background InformationProtein aurora borealis (UniProt Q6PGQ7; also known as HsBora) is encoded by the BORA (also known as C13orf34) gene (Gene ID 79866) in human. Originally identified as a co-factor for Aurora A-dependent asymmetrical cell divisions, Bora is an adaptor/scaffold protein that plays an important role in mitosis by mediating Aurora A-dependent Plk1 activation, and Bora depletion abolishes both Plk1 and Cdk1 activation. Cellular Bora activity is negarively regulated by Plk1- and Cdk1-mediated phosphorylation. Cdk1 initially phosphorylates Bora on S252, which primes Bora for Plk1-catalyzed S497 and T501 phosphorylation, leading to SCFβTrCP-mediated Bora ubiquitination and subsequent proteasomal degradation. The negative feed-back regulation of Bora by the kinase it activates is important, as uncontrolled Bora overexpression perturbs Aurora A and Plk1 localization, leading to lengthened metaphase. Similar to the counter regulation between Bora and Cdk1/Plk1 in somatic cells, Bora degradation is reported to be regulated via phosphorylation by Cdk1 and Plx1 in xenopus ooctyes. Cdk1 is reported to phosphorylate Bora on Thr52, which blocks its degradation. Fertilization induced calcium surge jump-starts the oscillations of the embryonic cycles by inducing Calcineurin-catalyzed Bora pThr52 dephosphorylation, leading to Bora degradation.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Bora Antibody is an antibody against Bora for use in Western Blotting, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: A representative lot detected both the endogenous Bora and a FLAG-tagged Bora exogenously expressed in HEK293T cells. Downregulation of both the endogenous and FLAG-tagged Bora was seen following replication stress induction by camptothecin (Cat. No. 208925) or UV treatment of HEK293T cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
      Western Blotting Analysis: A representative lot detected FLAG-tagged wild-type and T501A Bora exogenously expressed in U2OS transfectants. Only the wild-type, but not the T501A mutant, was seen downregulated upon UV irradiation of the transfected U2OS cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
      Immunoprecipitation Analysis: A representative lot immunorecipitated significantly higher amount of ubiquitinated Bora from UV-irradiated HEK293T cells pre-treated with the proteasome inhibitor MG-132 than UV-treated cells without MG-132 (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
      Immunoprecipitation Analysis: A representative lot immunorecipitated Bora with enhanced Thr501 phosphorylation from UV irradiated HEK293T cells (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144).
      Biological Information
      ImmunogenGST-tagged recombinant human Bora N-terminal fragment.
      EpitopeN-terminal.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antiserum reacts with unmodified as well as postranslationally modified (ubiquitination and phosphorylation) Bora (Qin, B., et al. (2013). J. Biol. Chem. 288(22):16139-16144). Expected to react with both spliced isoforms of human Bora reported by UniProt (Q6PGQ7).
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BORA
      • C13orf34
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~58 kDa observed. 61.20 kDa (isoform 1) and 53.44 kDa (isoform 2) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: An 1:500 dilution of this antibody detected Bora in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1858 04055977330274