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AM03 Anti-Bak (Ab-1) Mouse mAb (TC-100)

This Anti-Bak (Ab-1) Mouse mAb (TC-100) is validated for use in Immunoblotting for the detection of Bak (Ab-1).

Anti-Bak (Ab-1) Mouse mAb (TC-100) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Bcl-2 Antagonist Killer

AM03
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Overview

Replacement Information

Key Spec Table

HostAntibody Type
MMonoclonal Antibody

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AM03-100UG
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      Description
      OverviewRecognizes the ~30 kDa (apparent MW) Bak protein in HL-60 cells.
      Catalogue NumberAM03
      Brand Family Calbiochem®
      SynonymsAnti-Bcl-2 Antagonist Killer
      Application Data
      Detection of human Bak by immunoblotting. Sample: Whole cell lysates from untreated HL-60 cells. Primary antibody: Anti-Bak (Ab-1) Mouse mAb (TC-100) (Cat. No. AM03) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesChittenden, T., et al. 1995. Nature 374, 733.
      Farrow, S.N., et al. 1995. Nature 374, 731.
      Kiefer, M.C., et al. 1995. Nature 736.
      Reed, J.C. 1994. J. Cell Biol. 124, 1.
      Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlHL-60 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ200
      Applications
      Application ReferencesImmunocytochemistry Panaretakis, T., et al. 2002. J. Biol. Chem. 277, 44317.
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence)
      Immunocytochemistry (see comments; see application references)
      Application CommentsThis antibody recognizes active/activated Bak under nondenaturing, native conditions using applications like immunocytochemistry (see application references). Antibody should be titrated for optimal results in individual systems for each application.
      Biological Information
      Immunogenrecombinant human Bak from which the C-terminal and transmembrane domains have been deleted
      ImmunogenHuman
      EpitopeWithin the N-terminal region
      CloneTC-100
      HostMouse
      IsotypeIgG2a
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AM03-100UG 04055977227970

      Documentation

      Anti-Bak (Ab-1) Mouse mAb (TC-100) Certificates of Analysis

      TitleLot Number
      AM03

      References

      Reference overview
      Chittenden, T., et al. 1995. Nature 374, 733.
      Farrow, S.N., et al. 1995. Nature 374, 731.
      Kiefer, M.C., et al. 1995. Nature 736.
      Reed, J.C. 1994. J. Cell Biol. 124, 1.
      Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure

      Citations

      Title
    • Lena Thyrell, et al. (2004) Interferon α-induced apoptosis in tumor cells is mediated through the phosphoinositide 3-kinase/mammalian target of rapamycin signaling pathways. Journal of Biological Chemistry 279, 24152-24162.
    • Aleksandra Mandic, et al. (2001) Cisplatin induces the proapoptotic conformation of Bak in a ΔMEKK1-dependent manner. Molecular and Cellular Biology 21, 3684-3691.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-August-2007 RFH
      SynonymsAnti-Bcl-2 Antagonist Killer
      ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence)
      Immunocytochemistry (see comments; see application references)
      Application Data
      Detection of human Bak by immunoblotting. Sample: Whole cell lysates from untreated HL-60 cells. Primary antibody: Anti-Bak (Ab-1) Mouse mAb (TC-100) (Cat. No. AM03) (2 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~30 (apparent MW) kDa Bak protein.
      BackgroundBak (for bcl-2 homologous antagonist/killer) is a member of the bcl-2 family of proteins. Bak was independently identified by three different groups using either the two hybrid system for identifying adenovirus E1B 19K interacting proteins or by PCR cloning using degenerate BH1 and BH2 domain primers derived from the human bcl-2 gene. Bak shows a 53% homology with bcl-2 in the BH1 and BH2 domains but an overall homology of 28% with bcl-2. The presence of a hydrophobic tail on the protein suggests that, like bcl-2, it is membrane localized. The Bak protein is translated from a 2.2 kb mRNA to yield a 211-216 amino acid protein with a predicted molecular weight of 23.4 kDa but migrates by gel electrophoresis as an approximately 30 kDa protein under denaturing conditions. The bak gene (bak-1) has been mapped to chromosome 6 and spans approximately 6 kb of DNA. Southern analysis has indicated the presence of two additional bak genes: bak-2 has 97% homology with bak-1 and maps to chromosome 20, and bak-3, a pseudogene, which maps to chromosome 11. Functionally, bak is similar to bax in that it induces apoptosis. The protein pairs with bcl-xL but unlike its pairing partner, has been found in most tissues tested. While conflicting reports exist for the heterodimerization of bak with other members of the bcl-2 family of proteins, it is clear that bak does partner with the E1B 19K protein.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human Bak from which the C-terminal and transmembrane domains have been deleted
      EpitopeWithin the N-terminal region
      CloneTC-100
      IsotypeIgG2a
      Specieshuman, not mouse, not rat
      Positive controlHL-60 cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody recognizes active/activated Bak under nondenaturing, native conditions using applications like immunocytochemistry (see application references). Antibody should be titrated for optimal results in individual systems for each application.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesChittenden, T., et al. 1995. Nature 374, 733.
      Farrow, S.N., et al. 1995. Nature 374, 731.
      Kiefer, M.C., et al. 1995. Nature 736.
      Reed, J.C. 1994. J. Cell Biol. 124, 1.
      Korsmeyer, S.J., et al. 1993. N. Semin. Cancer Biol. 4, 327.
      Citation
    • Lena Thyrell, et al. (2004) Interferon α-induced apoptosis in tumor cells is mediated through the phosphoinositide 3-kinase/mammalian target of rapamycin signaling pathways. Journal of Biological Chemistry 279, 24152-24162.
    • Aleksandra Mandic, et al. (2001) Cisplatin induces the proapoptotic conformation of Bak in a ΔMEKK1-dependent manner. Molecular and Cellular Biology 21, 3684-3691.
      		•
      Application referencesImmunocytochemistry Panaretakis, T., et al. 2002. J. Biol. Chem. 277, 44317.