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MAB3494 Anti-ATP Synthase Antibody, β chain, clone 4.3E8.D1

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MAB3494
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3494
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-ATP Synthase Antibody, β chain, clone 4.3E8.D1
      Alternate Names
      • Mitochondrial Marker
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-ATP Synthase Antibody, β chain, clone 4.3E8.D1 detects level of ATP Synthase & has been published & validated for use in IC & IP.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunocytochemistry: 1 μg/mL for one hour at room temperature using 10xE6 cells or adherent sample by indirect immunofluorescence. Suggested fixative is 2-4% paraformaldehyde. Suggested permeabilization method is 0.5% Triton X-100.

      Immunoprecipitation

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRat brain mitochondria
      Epitopebeta chain
      Clone4.3E8.D1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes the beta chain of ATP Synthase. The beta chain of ATP Synthase is a useful subcellular marker for mitochondria.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
      Gene Symbol
      • ATP5B
      • ATPMB
      • ATPSB
      • MGC5231
      • EC 3.6.3.14
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06576 # Produces ATP from ADP in the presence of a proton gradient across the membrane. The beta chain is the catalytic subunit.
      SIZE: 529 amino acids; 56560 Da
      SUBUNIT: F-type ATPases have 2 components, CF(1) - the catalytic core - and CF(0) - the membrane proton channel. CF(1) has five subunits: alpha(3), beta(3), gamma(1), delta(1), epsilon(1). CF(0) has three main subunits: a, b and c.
      SUBCELLULAR LOCATION: Mitochondrion.
      SIMILARITY: SwissProt: P06576 ## Belongs to the ATPase alpha/beta chains family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3494 04053252466731