Millipore Sigma Vibrant Logo

UFC5030BK Amicon Ultra-0.5 Centrifugal Filter Unit

View Products on Sigmaaldrich.com
UFC5030BK
500  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Fulfillment and delivery delayed
Fulfillment and delivery delayed
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsNMWLVolumeMinimum Final Concentrate Volume
      PExt, Protein Purification30 KDa0.5 mL15 µL
      Description
      Catalogue NumberUFC5030BK
      Replaces42411
      Trade Name
      • Amicon Ultra
      DescriptionAmicon Ultra-0.5 Centrifugal Filter Unit
      Background InformationAMICON ULTRA 0.5ML - 30KDa cutoff. Available in 4 pack sizes.
      UFC503008 - 8 pack
      UFC503024 - 24 pack
      UFC503096 - 96 pack
      UFC5030BK - 500 pack


      Concentrates 500 µL down to 15 µL
      Concentration factor 25 x to 30 x
      High sample recovery (greater than 90% of dilute starting solution)
      Fast processing time - 10 to 30 minutes
      Reverse spin capability provides consistent recoveries

      Applications:
      Concentration of biological samples containing antigens, antibodies, enzymes, nucleic acids, or microorganisms
      Purification of macromolecular components found in tissue culture extracts or cell lysates
      Primer removal, PCR cleanup, removal of linkers or macromolecular labels from a reaction mix, and protein removal prior to HPLC
      Desalting, buffer exchange and protein dialysis

      Material Supplied:
      The Amicon Ultra-0.5 device is supplied with two microcentrifuge tubes. During operation, one tube is used to collect filtrate; the other tube is to recover the concentrated sample.

      Performance – DNA Concentration:
      The Amicon Ultra-0.5 device provides the best balance between recovery and spin time for double-stranded DNA for base pairs ranging from 137 to 1159. To achieve maximum PCR product recovery and primer removal with primers greater than 20 bases, one or two additional spins with Tris-EDTA (TE) buffer are recommended.
      References
      Product Information
      Filter CodePLCGC
      Color CodeClear
      Filter TypeUltrafiltration
      NMWL30 KDa
      Quality LevelMQ400
      Applications
      ApplicationUltracel-30 regenerated cellulose membrane, 0.5 mL sample volume
      Key Applications
      • Protein Extraction
      • Protein Purification
      Biological Information
      SterilityNon-Sterile
      Physicochemical Information
      Centrifugal ApplicationProtein Concentration and Desalting
      Dimensions
      Length4.99 cm (1.96 in.)
      Diameter10.8 mm
      Filtration Area1 cm²
      Minimum Final Concentrate Volume15 µL
      Volume0.5 mL
      Materials Information
      Chemistry
      • Regenerated Cellulose (RC)
      Device Material
      • Housing: Styrene/butadiene
      • Cap and Tube: Polypropylene
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Material Size500
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      UFC5030BK 04053252664656

      Related Products & Applications

      Product Families

      Related Products By: Brand Facete

      Categories

      Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units