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324355 n-Dodecyl-β-D-maltoside, ULTROL® Grade - CAS 69227-93-6 - Calbiochem

CAS 69227-93-6 is a non-ionic detergent similar to n-octyl-β-D-glucopyranoside; however, increased alkyl chain length has been shown to increase thermal stability of lipid-free rhodopsin in solution.

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      Übersicht

      Replacement Information

      Key Spec Table

      CAS #
      69227-93-6
      Description
      OverviewNon-ionic detergent similar to n-octyl-β-D-glucopyranoside; however, increased alkyl chain length has been shown to increase thermal stability of lipid-free rhodopsin in solution. Useful in membrane-protein solubilization studies. Aggregation number: 98.
      Absorbance (10%, H2O, 260 nm): <1.0.
      Catalogue Number324355
      Brand Family Calbiochem®
      References
      ReferencesWitzmann, F., et al. 1991. Electrophoresis 12, 687.
      Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem. 171, 330.
      Rosevear, P., et al. 1980. Biochemistry 19, 4108.
      Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids 23, 321.
      Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta 425, 46.
      Product Information
      CAS number69227-93-6
      Aggregation Number98
      CMC100-600 µM
      FormWhite solid
      Hygroscopic Hygroscopic
      Micellar weight50000
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥98% by HPLC
      Physicochemical Information
      Contaminantsdodecanol 1H NMR: ≤1%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C).
      Stock solutions are stable for up to 3 months at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      324355 0

      Documentation

      n-Dodecyl-β-D-maltoside, ULTROL® Grade - CAS 69227-93-6 - Calbiochem SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      n-Dodecyl-β-D-maltoside, ULTROL® Grade - CAS 69227-93-6 - Calbiochem Analysenzertifikate

      TitelChargennummer
      324355

      Literatur

      Übersicht
      Witzmann, F., et al. 1991. Electrophoresis 12, 687.
      Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem. 171, 330.
      Rosevear, P., et al. 1980. Biochemistry 19, 4108.
      Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids 23, 321.
      Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta 425, 46.

      Broschüre

      Titel
      Biological Detergents Brochure
      CALBIOSORBﾙ Technical Bulletin

      Literaturstellen

      Titel
    • Serban P. Georgescu, et al. (2005) Modulator recognition factor 1 (MRF1), an ARID-family member, is a novel co-repressor for estrogen receptor α. Molecular Endocrinology 19, 2491-2501.
      		•
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-December-2010 JSW
      DescriptionA water-soluble nonionic detergent. Studies on rhodopsin have shown that alkyl glycosides with longer alkyl chains are better at preserving the protein's properties. In addition, dodecyl maltoside's longer hydrophilic group confers higher solubility in water. This is in contrast to undecyl and dodecyl glucosides, which have low solubility in water. Due to its low CMC, dodecyl maltoside is not easily removed by dialysis. The half time for dialysis is 20 h. This detergent is milder than nonyl glucoside. Of several glycosides tested, dodecyl maltoside was found to be the most useful for the preparation of active cytochrome oxidase from Neurospora. In octyl glucoside, cytochrome oxidase dissociated into its subunits and became inactive. Similarly, beef heart oxidase when equilibrated in octyl glucoside formed a mixture of polymer, dimer, and lower molecular weight forms and underwent denaturation. In contrast, the enzyme, when equilibrated in dodecyl maltoside, was found to exist as a uniform species of 30 kDa, which is consistent with an active dimeric form. Dodecyl maltoside has a critical micelle concentration (CMC) of 100-600 µM and a micellar weight of 70,000. Aggregation number: 98.
      FormWhite solid
      CAS number69227-93-6
      Structure formulaStructure formula
      Purity≥98% by HPLC
      Contaminantsdodecanol 1H NMR: ≤1%
      SolubilityH₂O (100 mg/ml)
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, refrigerate (4°C).
      Stock solutions are stable for up to 3 months at 4°C.
      Toxicity Standard Handling
      ReferencesWitzmann, F., et al. 1991. Electrophoresis 12, 687.
      Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem. 171, 330.
      Rosevear, P., et al. 1980. Biochemistry 19, 4108.
      Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids 23, 321.
      Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta 425, 46.
      Citation
    • Serban P. Georgescu, et al. (2005) Modulator recognition factor 1 (MRF1), an ARID-family member, is a novel co-repressor for estrogen receptor α. Molecular Endocrinology 19, 2491-2501.
      		•