Millipore Sigma Vibrant Logo

507858 PANSORBIN® Cells

PANSORBIN® Cells: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Staphylococcus aureus Cells

View Products on Sigmaaldrich.com
507858
Preis & Verfügbarkeit

Übersicht

Replacement Information

Preis & Verfügbarkeit

Bestellnummer VerfügbarkeitVerpackung St./Pkg. Preis Menge
507858-10GM
Verfügbarkeit wird abgerufen...
—
Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      		 Glasflasche 10 gm
      Preis wird abgerufen...
      Preis nicht abrufbar
      Die Mindestmenge muss ein Vielfaches sein von
      Maximum Quantity is
      Bei Bestätigung Weitere Informationen
      Sie haben () gespart
       
      —
      Bitte erfragen
      		 —
      507858-1GM
      Verfügbarkeit wird abgerufen...
      —
      Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
      Lieferbar 
      Produkt wurde eingestellt
      Begrenzter Lagerbestand
      Bestätigung der Verfügbarkeit erforderlich
        Restmenge: Angebot folgt
          Restmenge: Angebot folgt
          Bitte erfragen
          Kontakt zum Kundenservice
          Contact Customer Service

          		 Glasflasche 1 gm
          Preis wird abgerufen...
          Preis nicht abrufbar
          Die Mindestmenge muss ein Vielfaches sein von
          Maximum Quantity is
          Bei Bestätigung Weitere Informationen
          Sie haben () gespart
           
          —
          Bitte erfragen
          		 —
          507858-50GM
          Verfügbarkeit wird abgerufen...
          —
          Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
          Lieferbar 
          Produkt wurde eingestellt
          Begrenzter Lagerbestand
          Bestätigung der Verfügbarkeit erforderlich
            Restmenge: Angebot folgt
              Restmenge: Angebot folgt
              Bitte erfragen
              Kontakt zum Kundenservice
              Contact Customer Service

              		 Glasflasche 50 gm
              Preis wird abgerufen...
              Preis nicht abrufbar
              Die Mindestmenge muss ein Vielfaches sein von
              Maximum Quantity is
              Bei Bestätigung Weitere Informationen
              Sie haben () gespart
               
              —
              Bitte erfragen
              		 —
              507858-5GM
              Verfügbarkeit wird abgerufen...
              —
              Eingeschränkte Verfügbarkeit Eingeschränkte Verfügbarkeit
              Lieferbar 
              Produkt wurde eingestellt
              Begrenzter Lagerbestand
              Bestätigung der Verfügbarkeit erforderlich
                Restmenge: Angebot folgt
                  Restmenge: Angebot folgt
                  Bitte erfragen
                  Kontakt zum Kundenservice
                  Contact Customer Service

                  		 Kst.-Ampulle 5 gm
                  Preis wird abgerufen...
                  Preis nicht abrufbar
                  Die Mindestmenge muss ein Vielfaches sein von
                  Maximum Quantity is
                  Bei Bestätigung Weitere Informationen
                  Sie haben () gespart
                   
                  —
                  Bitte erfragen
                  		 —
                  Description
                  Overview

                  PANSORBIN® Cells are heat-killed, formalin-fixed Staphylococcus aureus cells that have a coat of protein A and have been pickled by the method of Kessler. Useful for mitogenic stimulation of B lyphocytes and for immunoprecipitation.
                  Catalogue Number507858
                  Brand Family Calbiochem®
                  SynonymsStaphylococcus aureus Cells
                  References
                  ReferencesKierszenbaum, F., et al. 1991. Immunology 74, 317.
                  Meikle, P.J., et al. 1991. J. Biol. Chem. 266, 22569.
                  Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
                  Murakami, H., et al. 1988. Biochem. J. 256, 917.
                  Kessler, S.W. 1975. J. Immunol. 115, 1617.
                  Product Information
                  Activity≥2.0 mg human IgG/100 mg cells
                  FormHomogeneous, milky suspension
                  FormulationSupplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2.
                  Quality LevelMQ100
                  Applications
                  Key Applications Immunoprecipitation
                  Biological Information
                  Physicochemical Information
                  ContaminantsViable cells: ≤2500 cells/ml
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  Safety Information
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Ambient Temperature Only
                  Toxicity Standard Handling
                  Storage +2°C to +8°C
                  Do not freeze Yes
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications
                  Global Trade Item Number
                  Bestellnummer GTIN
                  507858-10GM 04055977272505
                  507858-1GM 04055977272499
                  507858-50GM 04055977271942
                  507858-5GM 04055977271935

                  Documentation

                  PANSORBIN® Cells SDB

                  Titel

                  Sicherheitsdatenblatt (SDB) 

                  PANSORBIN® Cells Analysenzertifikate

                  TitelChargennummer
                  507858

                  Literatur

                  Übersicht
                  Kierszenbaum, F., et al. 1991. Immunology 74, 317.
                  Meikle, P.J., et al. 1991. J. Biol. Chem. 266, 22569.
                  Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
                  Murakami, H., et al. 1988. Biochem. J. 256, 917.
                  Kessler, S.W. 1975. J. Immunol. 115, 1617.

                  Literaturstellen

                  Titel
                • Kira Steigerwald, et al. (2005) The APC tumor suppressor promotes transcription-independent apoptosis in vitro. Molecular Cancer Research 3, 78-89.
                  		•
                  Datenblatt

                  Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                  Revision06-May-2010 JSW
                  SynonymsStaphylococcus aureus Cells
                  DescriptionHeat-killed, formalin-fixed Staphylococcus aureus cells (Cowan I strain) that bear a high cell-surface density of protein A and have been pickled by the method of Kessler. Useful as a solid-phase IgG-binding reagent due to the high affinity interaction of protein A with the Fc domain of IgG. PANSORBIN® cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Most common applications include immunoprecipitation and mitogenic stimulation of B lymphocytes.
                  FormHomogeneous, milky suspension
                  FormulationSupplied as a 10% suspension of Staphylococcus aureus cells in PBS, 0.1% NaN₃, pH 7.2.
                  Recommended reaction conditions
                  The procedures used for performing immunoprecipitations using PANSORBIN® cells are somewhat variable, but this protocol can serve as a general guideline. PANSORBIN® cells work best when the antibody is human (IgG1, IgG2, IgG4), rabbit IgG (all isotypes), or mouse (IgG2a, IgG2b, IgG3). Prewashing: 1. Transfer desired amount of PANSORBIN® cells to a microfuge tube (typically 10-30 µl of a 10% PANSORBIN® cell suspension per immunoprecipitation). 2. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 3. Aspirate the supernatant. 4. Resuspend to the original volume or in 1.0 ml of cold lysis buffer, whichever is greater. Mix gently. 5. Repeat wash (steps 2-4). 6. Resuspend the final pellet to the original volume (from step 1) in lysis buffer. 7. If kept sterile, washed PANSORBIN® cells are stable at 4°C. Pre-Clearing (Optional): Pre-clearing with PANSORBIN® cells prior to immunoprecipitation removes material that binds nonspecifically, reducing the background. 1. Add 10-30 µl of washed PANSORBIN® cells to the lysate prior to incubation with the primary antibody. 2. Incubate for 1 h on ice, mixing occasionally. 3. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 4. Transfer the supernatant to another microfuge tube and discard the pellet. Alternatively, the lysate can be pre-cleared with PANSORBIN® cells which have been pre-bound to nonspecific antiserum (see Secondary Antibody pre-binding procedure). Primary Antibody: 1. Add the required amount of primary antibody to the lysate. 2. Incubate for at least 1 h on ice, mixing occasionally. 3. If a secondary antibody step is not required or desired, proceed to the PANSORBIN® Step described below. Secondary Antibody: In some cases, a secondary antibody will be necessary. For example, if the primary antibody is mouse IgG1, it is best to use a rabbit anti-mouse IgG as a secondary antibody. If desired, the secondary antibody can be pre-bound to the PANSORBIN® cells to eliminate the loss of antibody-antigen complexes which have not bound to the protein A. 1. Incubate the desired amount of secondary antibody with prewashed PANSORBIN® cells for 45-60 min on ice. 2. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 3. Aspirate the supernatant and wash the lysis buffer. 4. Resuspend in lysis buffer to a volume equal to the original volume of PANSORBIN® cells which were added. Alternatively, the unbound secondary antibody and PANSORBIN® cells can be incubated with the sample sequentially, following primary antibody incubation. PANSORBIN® Step: 1. Add at least 10X the amount of PANSORBIN® cells (alone or bound to secondary antibody) needed to precipitate the primary (or secondary) antibody. PANSORBIN® cells bind about 2 mg of human IgG/ml. Typically, add 10-30 µl of PANSORBIN® cells per immunoprecipitation. This quantity gives a visible pellet. 2. Incubate for 1-2 h on ice, mixing regularly. 3. Centrifuge at 3000 x g for 5 min, or at maximum speed in a microfuge for 1 min at 4°C. 4. Wash PANSORBIN® pellet 2-3 times with lysis buffer. 5. If samples are to be loaded onto a polyacrylamide gel, the PANSORBIN® pellet can be resuspended in sample buffer, boiled for 10 min, centrifuged quickly (30 s in a microfuge at 20°C). Load the supernatant onto the gel. If a high background is observed, it may be necessary to pre-clear lysates, prebind the antibody to PANSORBIN® cells, or use a stronger lysis buffer (e.g. RIPA). This protocol is meant to serve as a guideline and may need to be modified for specific applications.
                  ContaminantsViable cells: ≤2500 cells/ml
                  Activity≥2.0 mg human IgG/100 mg cells
                  Storage +2°C to +8°C
                  Do Not Freeze Yes
                  Toxicity Standard Handling
                  ReferencesKierszenbaum, F., et al. 1991. Immunology 74, 317.
                  Meikle, P.J., et al. 1991. J. Biol. Chem. 266, 22569.
                  Ezaki, O., et al. 1989. Biochem. Biophys. Res. Commun. 159, 1368.
                  Murakami, H., et al. 1988. Biochem. J. 256, 917.
                  Kessler, S.W. 1975. J. Immunol. 115, 1617.
                  Citation
                • Kira Steigerwald, et al. (2005) The APC tumor suppressor promotes transcription-independent apoptosis in vitro. Molecular Cancer Research 3, 78-89.
                  		•

                  Verwandte Produkte & Anwendungen

                  Alternative Products

                  Bestellnummer Beschreibung  
                  507861 PANSORBIN® Cells, Standardized Preis & Verfügbarkeit
                  507862 PANSORBIN® Cells, Lyophilized Preis & Verfügbarkeit

                  Related Products

                  Bestellnummer Beschreibung  
                  IP10 Protein G Plus/Protein A-Agarose Preis & Verfügbarkeit

                  Related Products

                  Bestellnummer Beschreibung  
                  507861 PANSORBIN® Cells, Standardized Preis & Verfügbarkeit

                  Verwandte Produkte nach: Brand Facete

                  PANSORBIN®

                  Kategorien

                  Life Science Research > Protein Detection and Quantification > Immunodetection Support Products > Protein A & Protein G Reagents