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FCMAB193F Milli-Mark™ Anti-CD33-FITC Antibody, clone WM-54

Milli-Mark Anti-CD33-FITC Antibody, clone WM-54 detects level of CD33 & has been published & validated for use in FC.

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology UniProt Number: P20138
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FCMAB193F
100 tests  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFCMFITCMonoclonal Antibody
      Description
      Catalogue NumberFCMAB193F
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark™ Anti-CD33-FITC Antibody, clone WM-54
      Alternate Names
      • Myeloid cell surface antigen CD33
      • Sialic acid-binding Ig-like lectin 3
      • Siglec-3
      • gp67
      Background InformationCD33 is a member of the siglec family (sialic acid binding immunoglobulin-like lectins) referred to as Siglec-3 (Freeman SD, Kelm S, Barber EK and Crocker PR, 1995, Blood, 2005-2012). It is the smallest member of the siglec family having a molecular weight of 67 kDa (Peiper SC, Ashmun RA and Look TA (1988) Blood., 72: 314-321). CD33 mediates protein-carbohydrate cellular interactions in addition to protein-protein recognition (Crocker, 2002). The CD33 gene is located on chromosome 19 (Peiper, 1997). CD33 is expressed by subsets of monocytes and neutrophils. CD33 is the major antigen to differentially diagnose myeloid from lymphoid leukemias, as it is expressed on 90% of myeloid and not lymphoid leukemic cells (Andrews 1989; Griffin 1984; and Sabbath 1986). Committed hemapoietic progenitors, including multipotential stem cells (CFU-GEMM) and precursors of granulocytes and macrophages (CFU-GM) and early erythroid progenitors (BFU-E) express CD33 (Peiper, 1989 and 1997). Anti-CD33, WM-54, was included in the Fourth International Workshop and Conference on Human Leucocyte Differentiation Antigens and studies by a number of laboratories confirmed its reactivity with CD33 (Peiper, 1989).
      References
      Product Information
      FormatFITC
      Control
      • PBMCs
      PresentationPurified Mouse monoclonal IgG1 conjugated to FITC in 0.05M Tris-HCl buffer with 1% BSA, Sodium Azide at 0.097% at pH 7.2.
      Quality LevelMQ100
      Applications
      ApplicationMilli-Mark Anti-CD33-FITC Antibody, clone WM-54 detects level of CD33 & has been published & validated for use in FC.
      Key Applications
      • Flow Cytometry
      Biological Information
      ImmunogenLeukemic cells from a patient with AML.
      CloneWM-54
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityAntibody recognizes Human CD33 antigen.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight67 kDa (Peiper, 1997)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by flow cytometry using human PBMCs
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintained refrigerated at 2-8°C from date of receipt. Protected from light in undiluted aliquots for 6 months from date of receipt.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      FCMAB193F 04053252289170

      Documentation

      Milli-Mark™ Anti-CD33-FITC Antibody, clone WM-54 SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Milli-Mark™ Anti-CD33-FITC Antibody, clone WM-54 Analysenzertifikate

      TitelChargennummer
      Milli-Mark Anti-CD33-FITC, clone WM-54 - 2151862 2151862
      Milli-Mark Anti-CD33-FITC, clone WM-54 - 2204952 2204952
      Milli-Mark Anti-CD33-FITC, clone WM-54 - NRG1761335 NRG1761335
      Milli-Mark™ Anti-CD33-FITC, clone WM-54 - 3733817 3733817
      Milli-Mark™ Anti-CD33-FITC, clone WM-54 -2514239 2514239
      Milli-Mark™ Anti-CD33-FITC, clone WM-54 -2694547 2694547
      Milli-Mark™ Anti-CD33-FITC, clone WM-54 Conjugate 3015366
      Milli-Mark™ Anti-CD33-FITC, clone WM-54 - 2390568 2390568
      Milli-Mark™ Anti-CD33-FITC, clone WM-54 - 2329152 2329152