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Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Gewähltes Kit
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96-Well Plate
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Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
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Autophagy, a degradative pathway that provides recycled nutrients to cells under stress, plays both protective and deleterious roles in many diseases, including cancer, neurodegeneration, and infections. Members of the LC3 family play a key role in the maturation of the autophagosome, the central organelle of autophagy. LC3 precursors, diffusely distributed in the cytosol, are proteolytically processed to form LC3-I. Upon initiation of autophagy, the C-terminal glycine is modified by addition of a phosphatidylethanolamine (PE) to form LC3-II, which translocates rapidly to nascent autophagosomes in a punctate distribution. DNA constructs encoding fluorescent proteins fused to LC3 are widely employed for introduction into cells for monitoring autophagosome formation by fluorescence microscopy. EMD Millipore’s LentiBrite™ GFP-LC3 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of autophagy in difficult-to-transfect cell types. The
LentiBrite™ GFP-LC3-II Enrichment Kit (Flow Cytometry) combines our GFP-LC3 lentiviral particles with a selective permeabilization procedure for GFP-LC3 transduced cells that allows release of cytosolic LC3-I and retention of autophagosome-bound LC3-II. This procedure allows detection of autophagosome density by flow cytometry.
Materials Required but Not Delivered
1. Test tubes for sample preparation, centrifugation and storage 2. Tissue culture reagents, i.e. HBSS, PBS w/o Ca2+ or Mg2+, cell dislodging buffers, etc. 3. Pipettors with corresponding tips capable of accurately measuring 10 – 1000 μL 4. V-bottom 96-well plates (Corning 2897) 5. Tabletop centrifuge capable of achieving 300 x g and holding 96-well plates 6. Mechanical vortex 7. Flow Cytometer 8. Deionized water (for reagent dilutions)
References
Product Information
Components
1. TagGFP2-LC3 Lentivirus: One vial containing 25 µL of lentiviral particles at a minimum of 3 x 10E8 infectious units (IFU) per mL. For lot-specific titer information, please see datasheet. • Virus Description: 3rd generation HIV-based VSV-G pseudotyped lentiviral particles. Lentiviral particles were generated using the pPACKH1 Lentivector Packaging System and purified by PEG precipitation. • Promoter: EF-1 (Elongation Factor-1). • Expression Marker Description: TagGFP2 (Evrogen) fused at its C-terminus with human LC3A (Accession # NP_115903) truncated to leave G120 (lipidation site) at the C-terminus. (Store at -80°C)
2. Autophagy Reagent A: One vial (lyophilized) (Store at 2-8°C) 3. 10x Enrichment Buffer (Part No. CS210552): One vial containing 1 mL (Store at 2-8°C) 4. 5x Assay Buffer: (Part No. CS210529): One bottle containing 3 mL (Store at 2-8°C)
EMD Millipore’s LentiBrite™ GFP-LC3 lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of autophagy in difficult-to-transfect cell types. The LentiBrite™ GFP-LC3-II Enrichment Kit (Flow Cytometry) combines our GFP-LC3 lentiviral particles with a selective permeabilization procedure for GFP-LC3 transduced cells that allows release of cytosolic LC3-I and retention of autophagosome-bound LC3-II. This procedure allows detection of autophagosome density by flow cytometry.
For optimal fluorescent analysis, it is recommended to analyze the target expression level within 24-48 hrs after transfection/infection for optimal live cell analysis, as fluorescent intensity may dim over time, especially in difficult-to-transfect cell lines. Infected cells may be frozen down after successful transfection/infection and thawed in culture to retain positive fluorescent expression beyond 24-48 hrs. Length and intensity of fluorescent expression varies between cell lines. Higher MOIs may be required for difficult-to-transfect cell lines.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store TagGFP2-LC3 Lentivirus at -80°C. After first thaw, place immediately on ice and freeze in working aliquots at -80°C. Frozen aliquots may be stored for at least 2 months. Further freeze/thaws may result in decreased virus titer and transduction efficiency.
Store Autophagy Reagent A, 10x Enrichment Buffer, and 5x Assay Buffer at 2-8°C.
All components are stable for at least 4 months from date of receipt when stored as recommended above.
