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14-1057 Human native nucleosomes

Human native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease & purified by glycerol gradient & ion exchange chromatography.

Human native nucleosomes: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Key Applications: Activity Assay
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14-1057
20 µg  
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      Replacement Information

      Key Spec Table

      Key Applications
      ACT
      Description
      Catalogue Number14-1057
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHuman native nucleosomes
      OverviewHuman native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease, and purified by glycerol gradient and ion exchange chromatography.
      Background InformationNucleosomes are the basic units of chromatin, and are comprised of approximately 147 bp of DNA spooled around a central octamer of core histones, H2A, H2B, H3, and H4. The arrangement of nucleosomes in chromatin can be altered by post-translational modifications (PTM) occurring on the C- and N-terminal tails of the core histones. Histone modifications can either provide or hinder access of key transcriptional complexes to DNA sequences and can regulate the expression of genes. These effects can be deciphered by applications such as chromatin immunoprecipiation (ChIP), which requires high quality chromatin, and essential tools such as histone proteins and appropriate enzymes, to assemble chromatin in vitro.
      References
      Product Information
      Presentation20 μg of mononucleosomes in buffer containing 20 mM Tris-Cl pH 7.9, 1 mM EDTA, 0.5 mM PMSF,1 mM DTT, and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationHuman native nucleosomes purified from HeLa cell nuclei, digested with micrococcal nuclease & purified by glycerol gradient & ion exchange chromatography.
      Key Applications
      • Activity Assay
      Application NotesSuitable for in vitro chromatin assembly and enzyme activity assays
      Biological Information
      Concentration0.2 µg/µL
      Purity>98% purity by SDS PAGE
      SourceHuman
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -80ºC for 1 year from date of receipt.
      Packaging Information
      Material Size20 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      14-1057 04053252694653

      Documentation

      Human native nucleosomes SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Human native nucleosomes Analysenzertifikate

      TitelChargennummer
      Human native nucleosomes - 2276397 2276397
      Human native nucleosomes - 2527355 2527355
      Human native nucleosomes - 2987717 2987717
      Human native nucleosomes - 3174013 3174013
      Human native nucleosomes - 3212332 3212332
      Human native nucleosomes - 3253395 3253395
      Human native nucleosomes - 3471938 3471938
      Human native nucleosomes - 3506084 3506084
      Human native nucleosomes - 3560695 3560695
      Human native nucleosomes - 3593887 3593887
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